These membraneless organelles in bacteria have been found to undergo Liquid-Liquid phase separation (LLPS), a molecular mechanism that allows for their assembly. Through extensive research, the occurrence of LLP
Using an optogenetic tool to interrogate protein properties associated with phase separation in living cells17,18, we identified surprisingly strong light-induced clustering of human RPA2 (Fig. 1a,b and Extended Data Fig. 1a–c). The prion-like N-terminal domain of FUS and an oligomerization-pr...
Carboxysomes in cyanobacteria enclose the enzymes Rubisco and carbonic anhydrase to optimize photosynthetic carbon fixation. Understanding carboxysome assembly has implications in agricultural biotechnology. Here we analyzed the role of the scaffolding protein CcmM of the β-cyanobacterium Synechococcus elongatus...
P-bodies mRNA decay RNase E liquid-liquid phase separation bacteria RNA degradation stress granules Introduction mRNA turnover provides an essential pathway in the control of gene expression across the domains of life. The most common bacterial protein controlling mRNA turnover is RNase E, which is...
To assess the phase separation of N-terminally His-tagged, full-length Bik1 we recombinantly expressed and purified the protein from bacteria (Bik1 FL; Fig. 1C, Supplementary Fig. 2A). The circular dichroism (CD) spectrum and thermal unfolding profile recorded at 222 nm for Bik1 FL were...
DEAD-box helicases are conserved from bacteria to mammals containing the N-terminal domain, helicase core domain, and C-terminal domain41. The interactions implicated in the phase separation include long-range electrostatic effects, prion-like property-mediated interactions, and aromatic amino acid-med...
Emerging evidence suggests that liquid–liquid phase separation (LLPS) represents a vital and ubiquitous phenomenon underlying the formation of membraneless organelles in eukaryotic cells (also known as biomolecular condensates or droplets). Recent studi
The present invention relates to methods and pharmaceutical reagents for decreasing the phase separation temperature and inhibiting the formation of high molecular weight aggregates in eye lenses, the
Charge-Patterned Disordered Peptides Tune Intracellular Phase Separation in Bacteria Subcellular phase-separated compartments, known as biomolecular condensates, play an important role in the spatiotemporal organization of cells. To underst... More by Allie C. Obermeyer,emailprotected,* Email: emailprotect...
Aqueous phase separation is employed to reduce or substantially eliminate bleed between adjacently-printed ink-jet ink compositions. An ink-jet ink set is provided that comprises first and second ink-