DEAD-box RNA helicases are evolutionarily conserved regulators of RNA-containing phase-separated organelles23, including nucleoli24. DDX18 was one of the 86 proteins whose depletion in the siRNA screen impacted nucleolar structure maintenance in HeLa cells25. By studying DDX18 in human ESCs (hESCs)...
The ability of proteins and nucleic acids to undergo liquid–liquid phase separation has recently emerged as an important molecular principle of how cells rapidly and reversibly compartmentalize their components into membrane-less organelles such as the
et al. Dead-box atpases are global regulators of phase-separated organelles. Nature 573, 144–148 (2019). Article ADS CAS PubMed Google Scholar Linsenmeier, M. et al. Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, rna and biochemical activity....
DEAD-box ATPases are global regulators of phase-separated organellesdoi:10.1038/s41586-019-1502-yMaria HondeleRuchika SachdevStephanie HeinrichJuan WangPascal VallottonBeatriz M. A. FontouraKarsten WeisNature Publishing Group
et al. DEAD-box ATPases are global regulators of phase-separated organelles. Nature 573, 144–148 (2019). Article CAS PubMed Google Scholar Hofweber, M. & Dormann, D. Friend or foe—post-translational modifications as regulators of phase separation and RNP granule dynamics. J. Biol. ...
Accumulating evidence suggests that liquid–liquid phase separation (LLPS) plays a crucial role in regulating the homeostasis and activity of ribonucleoprotein complexes, particularly the nonmembrane organelles known as stress granules [25–27]. The P150 isoform of hADAR1 localizes to and negatively re...
Intracellular membraneless organelles formed by liquid-liquid phase separation (LLPS) function in diverse physiological processes and have been linked to tumor-promoting properties. The nucleolus is one of the largest membraneless organelle formed through LLPS. Deubiquitylating enzymes (DUBs) emerge as ...
FG-Nups are highly mobile and can be found outside the NPC, where they may contribute to the formation of phase-separated membraneless organelles, such as SGs and processing bodies (P-bodies) [60,105]. To transport cargo across the NPC, NTRs must navigate through the permeability barrier ...
“Methods” section, Supplementary Data6). We complemented our predictions with an analysis of protein occurrence in phase-separated organelles36(Supplementary Data7). In agreement with our predictions, proteins binding to G4A4 in the presence of K+show enrichment across the condensation states of ...
Hondele M, Sachdev R, Heinrich S, Wang J, Vallotton P, Fontoura BMA, Weis K (2019) DEAD-box ATPases are global regulators of phase-separated organelles. Nature 573:144–148 Article CAS PubMed Google Scholar Huang R, Reusch RN (1996) Poly(3-hydroxybutyrate) is associated with specific...