Here, we describe a biomolecular interaction detection method, called phase-separated condensate-aided enrichment of biomolecular interactions in test tubes (CEBIT). The readout of CEBIT is the selective recruitment of biomolecules into phase-separated condensates harboring their cognate binding partners. ...
Furthermore, the molecular mechanisms by which regulatory factors nucleate on chromatin to assemble transcriptional condensates remain incompletely understood. The CBX-PRC1 complexes compartmentalize key developmental regulators for repression through phase-separated condensates driven by the chromobox 2 (CBX2)...
3d). We also tested whether the droplets were irreversible aggregates or reversible phase-separated condensates. To this end, GFP-Nur77 was allowed to form droplets in an initial solution, and then diluted sequentially by five times in equimolar protein concentration and crowding agent. The ...
RIM and RIM-BP form presynaptic active-zone-like condensates via phase separation. Mol. Cell 73, 971–984 (2019). CAS PubMed Google Scholar Banani, S. F. et al. Compositional control of phase-separated cellular bodies. Cell 166, 651–663 (2016). CAS PubMed PubMed Central Google ...
We demonstrate that, in comparison to T =0 , there is a suppression in the phase separation of the binary condensates at T ≠0 . This arises from the interaction of the condensate atoms with the thermal cloud. We also show that, when T ≠0 , it is possible to distinguish the phase-...
For macroscopically phase separated systems, the critical point can be found rapidly using the method of equal volumes (as in Fig. 3, see Methods - critical point measurement). However, this is impractical for most biologically relevant condensates, where only small volumes of condensed phases are...
相分离(phase separation, PS)是调控生物大分子(如蛋白质,核酸)在细胞中区室化分布的重要机制之一。根据参与相分离过程的不同机制可以将相分离蛋白划分为两类:一类能够自发组装形成相分离凝聚体(self-assembly);另一类则依赖与其他生物大分子的相互作...
As a control, HA-mNG-VP35 expressed alone phase separated in the cytoplasm (Fig.1c). Most cells have large quantities of small (sub-µm) VP35-containing dense phases (condensates) with high apparent mobility relative to the dilute phase, but a small number of condensates had a larger ...
Mechanistically, ABCF1 recognizes cccDNA by KKx4 motif and forms phase‐separated condensates by the poly‐glutamine (PolyQ) region of the N‐terminal intrinsically disordered low‐complexity domain (LCD). Subsequently, ABCF1‐cccDNA phase separation not only activates the type I/III interferon (IFN...
Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome Sequential metabolic enzymes have long been hypothesized to form multienzyme metabolic complexes to regulate metabolic flux in cells. Although in vitro bio... S An,M Jeon,EL Kennedy,... - 《Methods in ...