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Then, the membrane was blocked with protein-free rapid blocking buffer on a shaker for 10 min and washed with TBST 3 times, each time for 8 min. The membrane was incubated with the primary antibody (p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb, 1:1000, Cell Signaling; Phospho-p44/42 ...
More recently, an accurate analysis on the EGFR-21KR mutant was performed by mass spectrometry164 and it was found that this mutant still retained some ubiquitination, possibly responsible for the residual EGFR-CME internalization. Although these studies were carefully conducted, the caveat is that ...
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