We aimed to investigate the capacity of monocytes to produce tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) following IFN-γ stimulation and the associated role of IL-10 in TNF-α and IL-6 production.doi:10.2147/JIR.S245064Rona Kartika...
teetionofintraeellulareytokinesinperipheralbloodmononuclear cells:comparativeanalysisofphorbolmylistateacetate—ionomyeinand phytohemagglutininstimulation[J].ClinDiagnLabImmunol,2ool; 8(2):303-313. [收稿2005-08-09 修回2006-01-26] (编辑许四平) ...
Previously, we had observed in impaired response of UCBL to PHA and Con A stimulation in the presence of dialyzed human plasma (DHP). Our present results indicate that this impaired response is restored exclusively by TPRF. A factor with TPRF-like activity was also isolated from the plasma ...
Mechanism of phytohemagglutinin (PHA) action The stimulatory properties of PHA and Con A in short-term human lymphocyte cultures have been compared. PHA binds more strongly to the cells than does Con A. Much smaller quantities of PHA than of Con A suffice for maximal stimulation. T... K Lin...
In the literature it is also described how carnitine can reduce the blastogenesis of T lymphocytes caused by phytohemagglutinin (PHA). This effect was studied on human peripheral blood mononuclear cells (PBMCs) in healthy volunteers (Conti et al; Molecular and Cellular Biochemistry; 131: 1-8; ...
Longitudinal changes in these expression levels were measured after basophil stimulation by IgE-dependent or IgE-independent mechanisms and compared with patients' asthma status. Results: Spontaneous expression levels of CD203c were significantly higher on basophils from patients with asthma exacerbation than...
The in vitro IFN‐induced USP18 mRNA change (USP18IFN‐N) was measured via comparison of quantitative PCR‐determined USP18 transcription levels of BPMCs cultured with and without IFN stimulation. Either for virological (VR) or serological response (SR), the baseline USP18IFN‐N was ...
In conclusion, stimulation with α7nAChR agonist PHA 568487 dampens the inflammatory response in human PBMCs. This finding suggests that the anti-inflammatory properties of the α7nAChR may have a role in treating CAD. NEW & NOTEWORTHY The α7nAChR is an important regulator of inflammation; ...
A suspension of exposed and unexposed human PBMC (0.5 脳 10~6 cells/ml) prepared and cultured in RPMI-1640 supplemented with 10% FCS for 18 h in the presence or absence of PHA (5 渭g/ml) and LPS (10 ng/ml) which used for stimulation of IL-2, IL-4, TNF-alpha, IL-10 and ...
together activates multiple signalling pathways, which allows for broad stimulation of cytokines in mixed PBMC cultures =-=(2, 13, 14)-=-. A disadvantage of this method is that changes in the cellular constituents of the PBMC population, such as is seen in HIV infection, ongoing autoimmune ...