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Lanes 4, 5, 6, and 7 represent affected individuals with homozygous p.Gln778* alteration (IV:4, IV:5, IV:6, IV:7). The band indicates a molecular weight of B140 kDa. GAPDH is used as control (lower band). European Journal of Human Genetics Loss of function mutation in DIAPH1 AG ...
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在sgrna的情况下,上述引导序列可以进一步包括另外的核苷酸以例如与引导序列的3'端之后的以下示例性核苷酸序列形成sgrna:在5'到3'朝向上的guuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuuuu(seqidno:201)或guuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgag...
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