volume ratio n-hexane/ethyl acetate/ethanol/water (3:5:2:6, v/v/v/v) was used for pH-zone-refining countercurrent chromatography, in which 20mmol/L of trifluoroacetic acid was added in the organic phase as a retainer and 15mmol/L of ammonia was added to the aqueous phase as an ...
肉桂酸AR50gTrans-Cinnamic acid140-10-3桂皮酸pH标准缓冲溶液(pH=4.00) 柠檬酸钠500gTri-Sodium citrate6132-0 4-3柠檬酸三钠 乳酸丁酯LR100mlButyl lactate138-22-7羟基初油酸丁酯 三氟乙酸酐CP100mlTrifluoroacetic anhydride407-25-0三氟* 十八酸甘油酯CP500mlGlycerol monostearate31566-31-3单硬酯酸甘油酯...
Monomeric dimAβ was eluted in a gradient from 30% (v/v) to 36% acetonitrile in water and 0.1% (v/v) trifluoroacetic acid at 80 °C. DimAβ-containing fractions were pooled, lyophilized, dissolved in hexafluoroisopropanol (HFIP), aliquoted in 1 mg portions, lyophilized again, and ...
H-Rink Amide-ChemMatrix solid support resin was purchased from PCAS BioMatrix Inc., Diisopropylehtylamine (DIEA), Piperazine, N, N-dimethylformamide (DMF), dichlorometh- ane (DCM), trifluoroacetic acid (TFA), methanol, acetonitrile, dimethyl sulfoxide (DMSO), and Dulbecco's modified Eagle's ...
Several neutral glycosphingolipids were hydrogenated and subjected to trifluoroacetylation in trifluoroacetic acid/trifluoroacetic acid anhydride under conditions leading to complete exchange of the N-acetyl groups of GalNAc for N-trifluoroacetyl. The derivatized glycosphingolipids were analyzed for bindi...
A novel countercurrent chromatographic technique is described for the preparative separation-with excellent resolution-of up to multigram quantities of organic acids. This separation is accomplished by addition of a simple acid (e.g., trifluoroacetic acid) to the sample solution or stationary phase, fo...
The mobile phase consisted of acetonitrile and water (50:50, v/v) with 0.1% trifluoroacetic acid (TFA). These parameters for DOXY were determined using a spectrophotometer (TECAN) at 360 nm. 2.4.3. Drug release The drug release profiles of DTXL and DOXY were characterized using the ...
However, protonation of the basic nitrogen atoms by trifluoroacetic acid (TFA) in acetonitrile and in dichloromethane proceeds smoothly. The formation of the bipyridinium cation 1-H+ by protonation leads in acetonitrile solutions to distinct changes in the UV–Vis spectra. An additional absorption ...
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange...
Andreev1, Keykavous Parang2,3 &Yana K. Reshetnyak1 A series of cyclic peptides containing a number of tryptophan (W) and glutamic acid (E) residues were synthesized and evaluated as pH-sensitive agents for targeting of acidic tissue and pH-dependent cytoplasmic delivery of molecules...