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Next, a loading buffer (BioNTech, Mainz, Germany) was added to the protein samples, and the samples were boiled for 10 min. The proteins were separated by electrophoresis in 10% sodium dodecyl sulfate-polyacrylamide gels (electrophoresis instrument purchased from Bio-Rad) and then transferred to ...
VLPs were purified by a process that involved two steps: (i) ultracentrifugation through a 20% sucrose cushion in TE buffer (50 mM Tris-HCl, 0.5 mM EDTA, 0.2% Triton X-100, pH = 8.0) at 16,000 rpm in an SW40 rotor (Beckman, Pasadena, CA, USA) for 21 h at 10 ◦C; and (...
International Journal of Molecular Sciences Article Rat Aquaporin-5 Is pH-Gated Induced by Phosphorylation and Is Implicated in Oxidative Stress Claudia Rodrigues 1,2,†, Andreia Filipa Mósca 1,2,†, Ana Paula Martins 1,2, Tatiana Nobre 1,2, Catarina Prista 3, Fernando Antunes 4, Ana ...