pGEM®-T Vector Map and Sequence Reference Points ...9C. Sequence and Multi-Cloning Site of the pGEM®-T Easy Vector ...10D. pGEM®-T Easy Vector Map and Sequence Reference Points...116. General Considerations for PCR Cloning ...12A. PCR Product Purity......
-T Vector Map and Sequence Reference Points Figure 2. pGEM ® -T Vector circle map and sequence reference points. pGEM ® -T Vector sequence reference points: T7 RNA polymerase transcription initiation site 1 multiple cloning region 10–113 ...
-T asy Vector Systems are supplied with 2X apid Ligation Buff er. Ligation reactions using this buff er may be incubated for 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation. enerally, an overnight incubation at ...
pGEM -3Zf(+) Vector circle map and sequence reference points. The pGEM® ® -3Zf(+) and pGEM -3Zf(–) Vectors are identical except for the orientation of the f1 origin. Use the T7 Promoter Primer or pUC/M13 Forward Primer to sequence ssDNA produced by the pGEM®-3Zf(+) ...
-3Zf(+) Vector at –20°C. III. pGEM ® -3Zf(+) Vector Multiple Cloning Region and Circle Map Figure 1. pGEM ® -3Zf(+) Vector promoter and multiple cloning region sequence. The sequence shown corresponds to RNA synthesized by T7 RNA polymerase and ...