3.ProtocolforLigationsUsingthepGEM ® -TandpGEM ® -TEasyVectorsandthe2XRapidLigationBuffer..4 3.A.LigationProtocol...4 3.B.OptimizingInsert:VectorMolarRatios...
-T and pGEM ® -T Easy Vectors: • Cloning PCR products. • Construction of unidirectional nested deletions with the Erase-a-Base ® System. • Production of ssDNA. • Blue/white screening for recombinants. • In vitro transcription from dual-opposed promoters. (For protocol informa...
3.ProtocolforLigationsUsingthepGEM ® -TandpGEM ® -TEasyVectorsandthe2XRapidLigationBuffer..4 3.A.LigationProtocol...4 3.B.OptimizingInsert:VectorMolarRatios...
Promega Notes MagpGem-T and pGem-T Easy vector systems, instructions for use of procedures A1360, A1380, A3600, A3610, technical manual. Dostopno preko strani (20.04.2012) http://www.promega.com/resources/protocols/technical-manuals/0/pgem-t-and-pgem- t-easy-vector-systems-protocol/...
promega 公司的 pGEM-T Easy Vector T载体这个产品的详细的中文使用说明书 使用说明书2015-05-14 上传大小:415KB 所需:44积分/C币 猪传染性胃肠炎病毒纤突蛋白全基因的克隆与序列分析 (2007年) 采用RT-PCR和重组PCR扩增猪传染性胃肠炎病毒(Transmissible gastroenteritis virus of swine,TGEV)TSX毒株纤突蛋白(spi...
Protocol...11V.TransformationsUsingthepGEM®-TandpGEM®-TEasyVectorLigationReactions...12A.Protocol...
Protocol for Ligations Using the pGEM®-T and pGEM®-T Easy Vectors and the 2X Rapid Ligation Buffer...4A. Ligation Protocol ...4B. Optimizing Insert:Vector Molar Ratio ...54. Transformations Using the pGEM®-T and
The A-tailing protocol outlined in Figure 1 can be modified so that DNA fragments generated using restriction enzymes that leave a 3´ overhang can be cloned into T vectors such as the pGEM(R)-T and pGEM(R)-T Easy Vectors. The method outlined in Figure 3 uses the 3´- ->5´ ...
Aim : The goal of this study was to find a full design of the polymerase chain reaction (PCR) protocol for determining SARS-CoV-2 using In silico tools. The receptor-binding domain (RBD) of the spike protein is a crucial component. Materials and Methods : RBD interacts with ACE2 ...