PGC-1α gene was subsequently downregulated by siRNA in miR-217-downregulated breast cancer cells to examine its effect on cancer proliferation and cell-cycle progression. In addition, another downstream target gene of miR-217, DACH1, was further downregulated in breast cancer cells to investigate ...
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PGC-1alpha direct silencing with siRNA in control fibroblasts led to a similar loss of SOD2 response to oxidative stress as observed in FRDA fibroblasts. PGC-1alpha activation with the PPARgamma agonist (Pioglitazone) or with a cAMP-dependent protein kinase (AMPK) agonist (AICAR) restored ...
In contrast, down-regulation of PGC-1alpha levels by transfection with PGC-1alpha siRNA increased BACE1 expression. These effects appeared to be dependent on PPARgamma, because PGC-1alpha did not affect Abeta and BACE1 levels in N2a cells transfected with PPARgamma siRNA or in PPARgamma knock...
Conversely, transfection of EPCs with PGC-1-siRNA rescued EPCs premature senescence and up-regulated SIRT1 and decreased P53/P21 expression, correlating closely with the down-regulation of PGC-1 alpha itself. In addition, the combined stimuli induced up-regulation of PGC-1 alpha expression was ...
Furthermore, 15-HETE and PGC-1 alpha siRNA caused significant alterations in related mechanisms including caspase activity, mitochondrial membrane potential, and Bcl-2 expression. Taken together, these results provide the first evidence to confirm the importance of PGC-1 alpha in mediating the ...
Loss of PGC-1伪 (siRNA) in the microcirculation of ET subjects eliminates this vasodilatory robustness rendering vessels susceptible to blockade of H2O2 alone. Pharmacological activation of PGC-1伪 with alpha-lipoic acid in isolated microvessels from sedentary, overweight, and obese subjects increases ...
In contrast, SIRT1 or PGC-1alpha siRNA knockdown blunted the inhibitory effects of melatonin on Drp1 expression and mitochondrial fission. These data indicated that melatonin exerted its cardioprotective effects by reducing Drp1-mediated mitochondrial fission in a SIRT1/PGC-1alpha-dependent manner. ...
DHM treatment upregulated PGC-1 alpha and FNDC5 expression, enhanced energy metabolism, as evidenced by NMR-based metabonomics analysis, and partially abolished the suppressive effects of Pgc-1 alpha siRNA on FNDC5 expression. These results suggest that DHM can stimulate irisin secretion partially ...
miR-217PGC-1 alphaDACH1LentivirussiRNABackground: In this study, we explored the functional mechanism of PPARg co-activator 1-alpha (PGC-1 alpha) in regulating miR-217-mediated breast cancer development in vitro.Biomedicine & pharmacotherapy =: Biomedecine & pharmacotherapie...