Loss of PGC-1伪 (siRNA) in the microcirculation of ET subjects eliminates this vasodilatory robustness rendering vessels susceptible to blockade of H2O2 alone. Pharmacological activation of PGC-1伪 with alpha-lipoic acid in isolated microvessels from sedentary, overweight, and obese sub...
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In contrast, SIRT1 or PGC-1alpha siRNA knockdown blunted the inhibitory effects of melatonin on Drp1 expression and mitochondrial fission. These data indicated that melatonin exerted its cardioprotective effects by reducing Drp1-mediated mitochondrial fission in a SIRT1/PGC-1alpha-dependent manner. ...
By using the siRNA (small interfering RNA) knockdown approach, we discovered that ERRgamma was involved in the initial phase, but not in the later phase, of PGC-1alpha-induced ERRalpha expression. 展开 关键词: Kidney Cell Line Chromatin Nucleosomes Animals Humans RNA Polymerase II Heat-Shock ...
Conversely, transfection of EPCs with PGC-1-siRNA rescued EPCs premature senescence and up-regulated SIRT1 and decreased P53/P21 expression, correlating closely with the down-regulation of PGC-1 alpha itself. In addition, the combined stimuli induced up-regulation of PGC-1 alpha expression was ...
Furthermore, 15-HETE and PGC-1 alpha siRNA caused significant alterations in related mechanisms including caspase activity, mitochondrial membrane potential, and Bcl-2 expression. Taken together, these results provide the first evidence to confirm the importance of PGC-1 alpha in mediating the ...
To determine whether a stimulated increase in PGC-1α levels using alpha-lipoic acid (ALA) can restore NO as the mediator of dilation in CAD vessels.Methods: non-CAD and CAD human adipose arterioles were incubated overnight with either siRNA to PGC-1α or ALA, an over-the-counter supplemen...
Moreover, Sirtinol and SIRT1 siRNA each blocked the melatonin-mediated elevation in mitochondrial function by inhibiting SIRT1/ PGC-1 alpha signaling. Luzindole, a melatonin receptor antagonist, was found to partially block the ability of melatonin to promote SIRT1/ PGC-1 alpha signaling. In ...
Secondly, by using siRNA, the inhibitory effect of E(2) on VSMC is strongly reduced via suppressing expression, indicating that E(2) may exert its role through restoring . Finally, E(2) (10nM) treatment inhibits OA-induced /2 (/2) , however, suppression of expression abolishes this ...
After treating cells with bortezomib or dexamethasone, the expression of PGC-1 alpha, superoxide dismutase 2 (SOD-2) and catalase (CAT) was examined by RT-PCR. PGC-1 alpha expression was also analyzed by western blotting. Small-interference RNA (siRNA) was applied to inhibit the expression ...