1 Petagram of carbon = 1 PgC = 1015 gramsof carbon= 1 Gigatonne of carbon = 1 GtC. Thiscor...
GAPDH GTCTTCCTTGAGCACCTGGATC GTCTTCCTTGAGCACCTGGATC 2.3. Western blotting BV2 cells were washed and lysed in RIPA lysis buffer (50 mM Tris–HCl, pH 8.0; 1% NP-40; 0.5% sodium deoxycholate; 150 mM NaCl; 0.1% SDS) containing protease and phosphatase inhibitor cocktails and 1 mM phenyl-meth...
Genotyping was performed with mouse tail DNA and primers of (ggctggtaccttggaactga) and (aatccgtcttcatccacagg). Two separate PGC-1α EC TG mouse lines were used for experiments. The PGC-1α EC TG mice were bred onto the background of eNOS−/− mice (Jackson Laboratory) to generate ...
G6PC, GCN5, PPARGC1A and CDK2 were designed using the PrimerBank (https://pga.mgh.harvard.edu/primerbank/) and corresponding primers were listed as follows: CCNE1 (forward, AAG GAG CGG GAC ACC ATG A; reverse, ACG GTC ACG TTT GCC TTC C), G6PC (forward, CTA CTA CAG CAA ...
ERR-α (Essra) 5′-CCAGGCTTCTCCTCACTGTC-3′ 5′-GCCCCCTCTTCATCTAGGAC-3′ TFAM (Tfam) 5′-AGCCAGGTCCAGCTCACTAA-3′ 5′-AAACCCAAGAAAGCATGTGG-3′ NRF-1 (Nrf1) 5′-TGGTCCAGAGAGTGCTTGTG-3′ 5′-TTCCTGGGAAGGGAGAAGAT-3′ NRF-2 (Nrf2) 5′-GGCTCGTCGACTGGAAGGCT-3′ 5′-TCTCCCC...
ggcttctttggctatgacaccctgactgtccagagcatccaggtccccaaccaggagttc ggcttgagtgagaatgagcctggtaccaacttcgtctatgcgcagtttgatggcatcatg ggcctggcctaccctgctctgtccgtggatgaggccaccacagctatgcagggcatggtg caggagggcgccctcaccagccccgtcttcagcgtctacctcagcaaccagcagggctcc agcgggggagcggttgtctttgggggtgtggatagcagcctgtacacggggcagatc...
To amplify different promoter regions, the corresponding forward primers 5′ -ACATTGGGT ACCGACTCTGCTGTAAGAAGGCCGGCA-3′ pSIRT3-636-Luc, 5′ - ATTCTCGGTACCCGCCTACTC AAGGAGGTCGAGAGC-3′ pSIRT3-250-Luc were used with the identical reverse primer 5′ -ATTCAGCTCGAGCCCACAGTCTGA...
(forward) and5′-CCACAGCGTCATATCATCCAG-3′ (reverse); PGC-1α: 5′-TATGGAGTGACATAGAGTGTGCT-3′(forward) and 5′-GTCGCTACACCACTTCA. ATCC-3′(reverse); GAPDH:5′-ACTTTGGCATCGTGGAAGGG-3′(forward) and 5′-ACTTGGCAGGTTTC. TCCAGG-3′(reverse). The amplification conditions were as ...
0061 AGGTACCGCT TCGGCGACTT CAGCGTGGCC TTGGAGCCCA TGGCCTACTT GGAAGCTGCC 0121 TACTTTGGCG AGATCAGCAT CGGGACCCCA CCACAGAACT TCCTGGTCCT TTTTGACACC 0181 GGCTCCTCCA ATCTGTGGGT GCCCTCTGTC TACTGCAAGA GCCTGGCCTG CACCACCCAT 0241 GCCCGCTTCA ACCCCAGCAA GTCCTCTACC TACTCCACCG ACAGGCAGAC CTTCTCCCTG ...
有趣的是,这个消息在GTC大会前一晚以保密新闻稿的形式发给了在场媒体,第二天的正式会议上却删掉了这一环节。 400亿美元收购Arm,这是一笔实打实的豪赌。英伟达过去十年间的净利润加起来都还不到交易额的一半。 当前,英伟达主宰着高性能计算市场,Arm垄断着低功耗计算市场,全球手机芯片与IoT芯片超过90%使用了Arm的架...