To enable rational antigen design, we solved a cryo-EM structure of the highly potent anti-PfCSP antibody L9 in complex with recombinant PfCSP. We found that L9 Fab binds multivalently to the minor (NPNV) repeat domain, which is stabilized by a unique set of affinity-matured homotypic, ...
L9 utilizes a distinct paratope structure to confer NPNV selectivity In the cryo-EM structure, each L9 Fab primarily engages a single NPNV repeat, while the DPNA repeats are largely unbound and serve as a linker between each NPNV (Fig.1e, g). This binding site model is strongly supporte...
Structure-function analysis revealed multiple somatic hypermutations that combinatorically improved protection. This mouse model can thus be used to understand vaccine immunogens and to develop highly potent anti-malarial antibodies.doi:10.1016/j.immuni.2021.10.017Kratochvil, Sven...