最近将测序正确的pFast bac重组质粒转化DH10 bac 感受态细胞，稀释到10-5,10-6 涂含有卡那霉素，...
The invention discloses a method for expression of a PCV 2 (Porcine circovirus type2) Cap protein by a pFast Bac Dual baculovirus. The method comprises the following steps of: amplifying a gene fragment of an encoded PCV 2 Cap protein with a His tag; connecting the gene fragment to a ...
技术领域本发明属于基因工程领域,具体涉及一种利用pFast Bac Dual杆状病毒表达猪圆环病毒2型(PCV2) Cap蛋白的方法。背景技术猪圆环病毒2型(Porcine circovirus type2,PCV2)是近年来新发现的一种重要病原微生物,该病毒被认为是断乳仔猪多系统衰竭综合征(Post-weaning multisystemicwasting syndrome, PMWS)的主要病原(...