1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control...