1.载体(Vector)的概念 把一个目的基因通过基因工程手段送到生物细胞(受体细胞),需要运载工具(交通工具)携带外源基因进入受体细胞, 这种运载工具就叫做载体(vector)。 基因工程上所用的载体是一类能自我复制的 DNA 分子,其中的一段 DNA 被切除而不影响其复制,可用以置换或插入 ...
Das Champion™ pET SUMO Expressionssystem produziert die höchsten Anteile an löslichem Protein in E. coli. Für verbesserte Löslichkeit vonexprimiertenWeitere Informationen Have Questions? KatalognummerK30001 Preis (EUR)/ Each 1.240,00 ...
pET His6 Sumo TEV LIC cloning vector (1S)质粒 货号:#29662-20ul 货号产品名称规格库存价格数量购买 #29662-20ulpET His6 Sumo TEV LIC cloning vector (1S)质粒20ul 立即咨询 +- 产品介绍 相关产品 》产品信息 货号名称规格 #29662pET His6 Sumo TEV LIC cloning vector (1S)质粒 ...
名称:pET His6 Sumo TEV LIC cloning vector (2S-T) 别称:Plasmid#29711 基因长: 规格:少量干粉质粒(现货)大提液体质粒(一周) 质粒宿主:大肠杆菌 质粒用途:蛋白表达 片段类型:ORF 片段物种:空载体 原核抗性:Amp 筛选标记: 荧光标记: 启动子:T7
Kras4BG12V oncogene was cloned into pET SUMO vector, followed by a 12 h chemically induced protein expression in Escherichia coli at 20掳C. Native Kras4BG12V protein was produced in a series of protein purification steps involving immobilised nickel ion-affinity column chromatography, SUMO fusion ...
pET-28a(+)-sumo 载体信息 产品货号GS-2227 销售价格￥ 1020 包装规格2μg质粒或0.5ml甘油菌 载体抗性Kanamycin 载体大小5633 bp 启动子T7/lac 5'测序引物T7: 5'-TAATACGACTCACTATAGGG-3' pET-28a(+)-sumo 载体图谱 相关载体推荐
each vector contains only the necessary functional elements and an N-or C-terminal 6xHis tag(pET300/NT-DEST and pET301/CT-DEST,respectively)for convenient purification and detection.The vector kit is ideal for structural biologists who desire no or minimal modifications to their protein of ...
pET-3c-sumo 载体信息 产品货号GS-2226 销售价格￥ 680 包装规格2μg质粒或0.5ml甘油菌 表达类型高 载体抗性Ampicillin (氨苄青霉素) 5'测序引物T7:5'-TAATACGACTCACTATAGGG-3'
Methods:The SUMO-FGFR4 fusion gene was obtained by Overlap PCR and was connected to pET22b;the recombinant expression vector pET22b-SUMO-FGFR4 was obtained. The influence of lactose concentration, induction time,induction temperature,induction point and adding mode of lactose in the expressionlevels...
Kras4BG12V oncogene was cloned into pET SUMO vector, followed by a 12 h chemically induced protein expression in Escherichia coli at 20?掳C. Native Kras4BG12V protein was produced in a series of protein purification steps involving immobilised nickel ion-affinity column chromatography, SUMO fusion ...