The collected fractions were diluted with water (total volume = 40 mL), passed through a Sep-Pak® C18 Plus cartridge (Waters, Milford, MA, USA; pre-conditioned with 5 mL of absolute EtOH and 60 mL water), and eluted with 1.5 mL of absolute EtOH. Evaporation of the solvent at 70 ...
Other synthesis components were obtained as follows: sterile filters were acquired from MilliporeSigma (Burlington MA, USA); C18 Vac 1cc Sep-Paks were purchased from Waters Corporation (Milford MA, USA); Sep-Paks were flushed with 5 mL of ethanol followed by 10 mL of sterile water prior to...
n)18 F nuclear reaction. Isotopically enriched [18O]water (97% enrichment) was irradiated by an 18-MeV proton beam and trapped on an anion exchange resin (Sep-Pak Light Waters Accell Plus QMA Cartridge, Waters Corporation, Milford, MA, USA), which was pre-conditioned with a 1-M potass...