Call 137-649-32037 to buy the vector in China. pET-28a(+) sequence landmarks Pvu I(4426) Sgf I(4426) Sma I(4300) Kan ( 399 5-4 80 7) T7 promoter T7 transcription start His?Tag coding sequence T7?Tag coding sequence Multiple cloning sites (BamH I - Xho I) His?Tag coding ...
Call 137-649-32037 to buy the vector in China. pET-28a(+) sequence landmarks Pvu I(4426) Sgf I(4426) Sma I(4300) Kan ( 399 5-4 80 7) T7 promoter T7 transcription start His Tag coding sequence T7 Tag coding sequence Multiple cloning sites (BamH I - Xho I) His Tag coding se...
A.DepartmentEBSCO_AspPlos OneSathiamoorthy S, Shin JA. Boundaries of the Origin of Replication: Creation of A pET-28a-derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors. PloS One. 2012; 7: 1-7. http://dx.doi.org/10.1371/journal.pone.0047259...
pET-28c(+) is a5367bp plasmid; subtract 2bp from each sitebeyond BamH I at 198. pET-28a-c(+) Vectorsl ac I (773-1852)or i (3286)Kan (3995-4807)f1 or ig in (4903-5358)Bpu1102 I (80)Xba I (335)Bgl II (401)SgrA I (442)Sph I (598)Mlu I (1123)Bcl I (1137)BstE ...
pET-28a(+)-sumo 载体信息 产品货号GS-2227 销售价格￥ 1020 包装规格2μg质粒或0.5ml甘油菌 载体抗性Kanamycin 载体大小5633 bp 启动子T7/lac 5'测序引物T7: 5'-TAATACGACTCACTATAGGG-3' pET-28a(+)-sumo 载体图谱 相关载体推荐
抗体纯化填料,耐碱重组蛋白A(Protein A) 面议 BL21(DE3)化学感受态细胞 ￥1350 产品简介 pET29a(+) 表达载体/vector:载体特点:强启动子,表达量高。赛尔瑞成(CRGEN)技术团队常年从事大肠杆菌原核表达重组蛋白制备工作,从而积累了pET系列、pGEX系列、pMAL系列等多种原核表达载体。同时,在这些常规表达载体的基础上,还...
Sathiamoorthy,Sarmitha,Shin,Jumi,A.摘要: During our studies involving protein-DNA interactions, we constructed plasmid pSAM to fulfill two requirements: 1) to facilitate transfer of cloned sequences from widely used expression vector pET-28a(+), and 2) to provide a vector compatible with pBR322...
pet28b,pet 28b载体价格800元,pET-28b载体质粒图谱(Vector map),载体序列(Sequence)和多克隆位点见下文,pet28b载体抗性为Kana,质粒大小为5368 bp,测序引物为T7和T7t,含有N-His, N-Thrombin, C-His标签,Novagen公司出品,主要用来大肠杆菌蛋白表达.
【Abstract】ObjectiveToconstructpET28aPTDAg85Bprokaryoticexpressionvector,andtoexploretheoptimizationofexpressionconditionsinE.coliBL21.MethodsUsingrestrictionenzymesNheIandBamHItodigestplasmidpET28aandtheninserttheTATPTDsequenceintoittoobtainpET28aPTDrecombinantplasmid,usingRTPCRforamplificationofAg85Bgene,andthencloning...
EF456735.1 Expression vector pET28-MHL, complete sequence 9376 9706 98% 0.0 99 参考资料：<a href="http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid=270" target="_blank" rel="nofollow noopener">http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid...