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IFNγ ELISpot assay The ELISpot assay was performed (FlowMetric) using the standard ELISpot protocol47and the human IFNγ single-color ELISpot kit (all reagents and plates included; stock keeping unit no. hIFNgp-1M, Cellular Technology Limited). Briefly, PBMCs from each patient (3 × 105...
O BHAYV (TX) -- {Can you say Austin Powers} OAKRAI (Youngstown, OH) -- {I'am an Oakland Raiders pro football fan(plates on my black 95 Camaro).} OICU812 (Fla) -- {Pizza delivery truck Oh I See You Ate One Too} OPBOOKS (NY) -- {Out of print books...our business and we...
chloro-3-indolyl phosphate/nitroblue tetrazolium as the substrate. ELISpot plates were analyzed on the CTL ImmunoSpot S6 Ultimate-V analyzer (Cellular Technology Limited) using ImmunoSpot software version 5.1. The cell viability and counts upon thawing of the PBMC vials are reported in Supplementary ...
The OCT interferogram is detected using a 1.6 GHz bandwidth high-speed double balanced photodetector (Thorlabs, PDB480C-AC) and a 5 GHz sampling rate super data sampling digital card (PXLe-5162, National Instruments, Austin, TX, USA). We placed a sliding guide rail at the "Target" ...
They can be divided into two main groups: scaffold-based spheroids, such as matrix on top, matrix embedded, matrix-encapsulation, spinner flasks and micropatterned plates; and scaffold-free spheroids, such as ultralow attachment plates, hanging drop, magnetic levitation, and magnetic 3D printing [...