Serial dilutions were made from the remaining 200 μL of the transformation product and spread on LB/ampicillin plates which were incubated overnight at 37°C, to calculate the library titer of unique clones the following day. Random clones were picked, checked for the presence of insert, and...
Microwave ablation has been widely accepted in treating bone tumor. However, its procedure is time-consuming and usually results in postoperative fractures. To solve this problem, we designed and fabricated titanium plates customized to the patients’ bone structures. The personalized titanium plates wer...
Multiscreen filter plates (Merck Millipore), precoated with antibodies specific for IFNγ (Mabtech), were washed with PBS and blocked with X-VIVO 15 (Lonza) containing 2% human serum albumin (CSL-Behring) for 1–5 h. Next, 3 × 105effector cells per well were stimulated for 16–2...
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An aliquot of fresh cells was taken on the day of collection and tested using the plate-based stimulation assay described above (24 h stimulation). Controls consisted of ‘mismatch’ peptide–HLA-coated plates. The supernatant was collected and analysed using IFNγ ELISA kits (Quantikine ELISA...
(class II type A2 biological safety cabinet/tissue culture hood; i.e., biosafety cabinet) with a class B surrounding environment. Microbial contamination was monitored using settle plates, volumetric active air sampling, and surface and operator sampling with contact plates. Continuous airborne ...
Ani- mal models of C9ALS repeat expansion have to be opti- mized and extensively characterized. However, based on clinical presentation, some specific characteristics are cen- tral features of C9 cases and should be found in animals. This includes RNA foci, DPR and TDP-43 positive aggre- ...
Furthermore, this non-precious-metal bar-based quality control element gains even more importance when it comes to more extended frameworks such as individual footplates with a higher number of screws and potential deformation during placement and rigid fixation. In conventional dental implant treatment...
A control series of plates were set up with samples not exposed to US in parallel. After 24 h, the areas of bacterial growth inhibition zones in both series of samples were counted (Figure 6b). The result of the experiment shows that US exposure has little effect on the minimum (1 wt ...
Cast immobilization is non-inferior to volar locking plates in relation to QuickDASH after one year in patients aged 65 years and older: a randomized controlled trial of displaced distal radius fractures. Bone Joint J. 2021;103-B(2):247-255. doi:10.1302/0301-620X.103B2.BJ...