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iPS cells were dissociated and centrifuged at 300gfor 3 min. Cells were resuspended in mTeSR1 media plus 10 μM ROCK inhibitor and single celled by gentle pipetting. Ten thousand cells were plated in a low-attachment 96-well plate, followed by centrifugation at 100...
Total microbial enumeration was performed using plate count [12] as it gives only viable bacteria. Standard total plate count (TPC) was done using TOS propionate agar (TPA) with mupirocin (Sigma-Aldrich (St. Louise, Missouri, USA)), under anaerobic conditions in triplicate, using the standard...