558050 Rev. 3 Page 1 of 2 Danger: BD Phosflow™ Perm Buffer III contains 87.68% methanol (w/w). Hazard statements Highly flammable liquid and vapor. Toxic if swallowed, in contact with skin or if inhaled. Causes damage to the central nervous system. Route of exposure: Oral. ...
1. Warm BD Phosflow™ Fix Buffer I (Cat. No. 557870) to 37 °C prior to use. 2. Chill BD Phosflow™ Perm Buffer III to -20 °C prior to use. 3. Collect PBMCs after Ficoll-Paque separation and treat with any appropriate cell activators as applicable. ...