Whole blood Buffy coat LRSC Leukopak® Erythrocytes Absolute numbers 5×109 cells/mL 4×1011 cells/buffy coat 5×1010 cells/LRSC 8×109 cells/half Leukopak® Ratio to PBMCs 2500 400 50 1.2 PBMCs Absolute numbers 2×106 cells/mL 1×109 cells/buffy coat 1×109 cells/L...
IFN纬, IL-7, and GM-CSF levels were higher in pre-treatment than in post-treatment samples of patients who developed irAEs but were lower in those without irAEs.#Baseline peripheral blood cytokine levels may predict irAEs in patients treated with immune checkpoint inhibitors and chemotherapy, ...
Leukopaks are enriched leukapheresis* products containing a variety of immune cells, including T cells, B cells, natural killer (NK) cells, monocytes, etc. Compared to whole blood and buffy coat, a full-size leukopak contains much higher concentrations of leukocytes per volume—typically greater ...
Isolate or Deplete Human Epithelial Cells from Whole Blood, Buffy Coat, Bone Marrow, MNC, Tissue Samples Isolation of Untouched Human B Cells by Depletion of Non-B Cells Isolation of Untouched Human CD4+ T Cells fr...
Blood 37:131–135, 1971. PubMed CAS Google Scholar Kurnick JE, Robinson WA. Colony growth of human peripheral white blood cells in vitro. Blood 37:136–141, 1971. PubMed CAS Google Scholar Goldman JM. Autografting cryopreserved buffy coat cells for chronic granulocytic leukaemia in ...
The method comprises the following steps: centrifuging the peripheral blood for 10 min under a condition of 2000rpm and 4 DEG C, and collecting the upper-layer plasma to prepare a serum-free medium containing the plasma; adding Ficoll separation liquid and centrifuging; sucking a buffy coat, ...
Nagaraj ER, Sharadadevi Mannur, Sastry AS. Laboratory diagnosis of malaria by conventional peripheral blood smears examined with Quantitative Buffy Coat (QBC) and Rapid Diagnostic Tests (RDT) - A comparative study - K, SastryBhatt S K, Sastry A S , Nagaraj E.R., Sharadadevi M, Sastry ...
and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. Samples from each donor are...
Baboon peripheral blood buffy coat cells were isolated, residual red blood cells were lysed with ammonium chloride buffer, and the cells were cultured at 5.0 × 104 per mL in 35-mm culture dishes (Nunc, Naperville, IL) using a two-layer agar culture technique as previously described in detail...
Tcell clonescan be used as either frozen pellets or harvested from culture.Peripheral blood mononuclear cellsare isolated from buffy coat preparations by centrifuging whole blood over Ficoll-Paque (Pharmacia). View chapter Book 1995,Recombinant DNA Methodology II ...