Free floating brain slices containing hypothalamus were washed (3 × 5 min) in 0.05 M tris buffered saline (TBS, pH 7.6) before a 15-min incubation in 0.3% H2O2 in TBS to block endogenous peroxidase activity. Next, sections were washed in TBS (3 × 5 min) and blocked in 1% bovine ...