adenylation–peptidyl carrier protein didomainreaction cycleMicrocystins, which are the most common cause of hepatotoxicity associated with cyanobacterial water blooms, are assembled in vivo on a large multienzyme complex via a mixed nonribosomal peptide synthetase/polyketide synthetase (NRPS/PKS). The ...
The first, the adenylation domain, selects and activates the monomer, transforming it into the adenylate form. The thiolation or peptidyl carrier protein domain covalently binds the activated monomer to the synthetase. The condensation domain catalyses the formation of a peptide bond between the ...
Finally, we have determined crystal structures of both apo- and holo-PA1221 proteins, the latter using a valine-adenosine vinylsulfonamide inhibitor that traps the adenylation domain-carrier domain interaction. The protein adopts an interface similar to that seen with the prior adenylation domain-...
The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for...
Central to the NRPS biosynthesis are peptidyl carrier protein (PCP) domains, small, catalytically inactive domains that shuttle substrates and intermediates between the catalytic modules and govern product release from TE domains. There is strong interest in recombination of NRPS systems to generate new...
产品介绍: 英文名称:L-allo-isoleucine:holo-[CmaA peptidyl-carrier protein] ligase 备注:This two-domain protein from the bacterium Pseudomonas syringae contains an adenylation domain (A domain) and a thiolation domain (T domain). It catalyses the adenylation of L-allo-isoleucine and its CB指数: ...
Fig. 2B shows that like the integral membrane protein Cox2, Pth3 and Pth4 were found uniquely in the membrane fraction, whereas Mrf1 partitioned between the membrane and soluble fraction. Thus Mrf1, Pth3 and Pth4 are all mitochondrial proteins, more-or-less tightly associated with the ...
(TIR) domain.DrosophilaToll and human TLR share homologous intracellular signaling components consisting of four major components: (1) the adaptor proteins MyD88, (2) TOLLIP (Toll-interacting protein), (3) the protein kinase IRAK (IL-IR-associated kinase), and (4) TRAF6 (TNF receptor-...
The Structure of PA1221 , a Non-Ribosomal Peptide Synthetase containing Adenylation and Peptidyl Carrier Protein Domains Supporting Information Synthesis of the Valine-adenosine vinylsulfonamide ( Val-AVS ). Figure S1 . Analysis of the valine binding pock...
Disclosed is a protein complex, comprising a physiologically active polypeptide, a dimeric protein and a non-peptidyl polymer having three functional ends (3-arm), with the linkage