In essence, the use of this Bayesian prior now favors scoring matrices that reflect amino acid similarity information encoded in PMBEC. Details of the derivation are shown in the following section. From here on, SMM refers to the original version of the method, where an identity matrix is ...
volume, and results in a high-quality MD model of the covalent complex. The validity of this model is confirmed using the experimental chemical shift data. The new MD-based approach offers a valuable tool to explore the mechanics of protein-peptide conjugation and build accurate models of covale...
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To illustrate this point, we have implemented a simple computational strategy based on rejection sampling (a simple version of Monte-Carlo sampling). In essence, we postulate that there is a certain probabilityp0that two proximal thiols would form a disulfide bond over the time interval of 1 ...
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The essence of LIVE-PAINT is to visualize individual fluorescent molecules transiently attached to a cellular structure of interest. The individual fluorophores are thus identified by temporal, rather than spatial, separation. LIVE-PAINT achieves sparse labeling by using reversible peptide–protein interacti...