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Direct protein sequencing technologies with improved sensitivity and throughput are still needed. Here, we propose an alternative method for peptide sequencing based on enzymatic cleavage and host–guest interaction-assisted nanopore sensing. We serendip
Saccharomyces cerevisiae MATa W303–1A strain was co-transformed with TIR1 and a β-estradiol-inducible Z4 zinc finger transcription factor (Z4EV) (52), whereas the MATα W814–29B strain was co-transformed with YFP-IAA7 or 28 and Z4-inducible CEP5pPro or mCEP5pPro following protocols ...
Tissue lysates were fractionated according to previous published protocols (Neumann et al., 2006). In brief, ~200mg tissue was homogenized in low salt buffer (10mM Tris, 5mM EDTA, 1mM DTT, 10% sucrose, 1x HALT protease/phosphatase inhibitor). Lysates were pelleted at 25,000 g for 30 min...
Haas BJ, Wortman JR, Ronning CM, Hannick LI, Smith RK, Maiti R, Chan AP, Yu C, Farzad M, Wu D, White O, Town CD: Complete reannotation of the Arabidopsis genome: methods, tools, protocols and the final release. BMC Biol. 2005, 3: 7-http://www.pubmedcentral.nih.gov/articlere...
eBook DRM-free (PDF, EPUB) now $60.95$60.95 Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manualreviews the literature and outlines the strategies for maximizing the successful appl...
Nuclear translocation of annexin A1 (ANXA1) has recently been reported to participate in neuronal apoptosis after cerebral ischemia. Prevention of the nuclear translocation of ANXA1 should therefore inhibit neuronal apoptosis and protect against cerebral
Next, the inventors asked whether free SUMO1 has the ability to influence the enzymatic activity of DPP9. The inventors tested this question by measuring GP-AMC hydrolysis by DPP9 in the presence of recombinant SUMO1. For control, activity was compared to samples containing DPP9 alone, or sam...
Furthermore, rapid and inexpensive selection protocols are available for identifying those library members that bind to a target of interest. Additionally, libraries displaying constrained, for example, cyclic, peptides can be distinguished based upon whether they display large single loop peptides, e....
and typically takes account of the disorder to be treated, the condition of the individual patient, the site of delivery, the method of administration and other factors known to practitioners. Examples of the techniques and protocols mentioned above can be found in Remington's Pharmaceutical Sciences...