the OSCs cloned in this study cluster into the following two main groups: (1) pentacyclic triterpene synthases and (2) cycloartenol synthases. Using the neighbour-joining method, the analysis showed that theMiFRS enzyme is a unique sequence and does not cluster with the friedelin synthase fromKala...
In this study, we determined the first cryogenic electron microscopy structure of a plant OSC, friedelin synthase, from Tripterygium wilfordii Hook. f (TwOSC). We also performed quantum mechanics/molecular mechanics simulations to reveal the energy profile for the CSR cascade reaction ...
This is an oleanane-type triterpene saponin from the underground parts oflicorice,Glycyrrhiza glabraL. This compound has some remarkable properties in the context of wound healing. Glycyrrhizin inhibits high-mobility group box 1 protein (HMGB1), secreted b...
The anti-inflammatory pentacyclic triterpene, oleanolic acid (OA) was examined on acute nociception induced by intraplantar injection of capsaicin in mice. OA administered orally to mice at 10, 30 and 100 mg kg−1, significantly attenuated the paw-licking response to capsaicin (1.6 μg/paw) ...
CcOSC5 encodes an enzyme for lupeol production as a single product. In conclusion, we identified various triterpenes and functionally characterized the triterpene synthase genes that participate in β-amyrin, α-amyrin, and lupeol biosynthesis in C. crenata....
Tingenone (200聽碌g/paw) administered into the right hind paw induced a local antinociceptive effect, that was antagonized by l-NOArg, nonselective nitric oxide synthase (NOS) inhibitor and by L-NPA, selective neuronal NOS (nNOS) inhibitor. The L-NIO, selective inhibitor of endothelial (eNOS...
Triterpene synthaseUrsolic acidCatharanthus roseus is an important medicinal plant and the sole commercial source of monoterpenoid indole alkaloids (MIA), anticancer compounds. Recently, triterpenoids like ursolic acid and oleanolic acid have also been found in considerable amounts in C. roseus leaf ...
ObAS1 was identified as a 尾-amyrin synthase, whereas ObAS2 was a mixed amyrin synthase that produced both a-amyrin and 伪-amyrin but had a product preference for 伪-amyrin. Moreover, transcript and metabolite analysis shed light on the spatiotemporal regulation of pentacyclic triterpene ...
The linear triterpene squalene, is derived from the reductive coupling of two molecules of farnesyl pyrophosphate Molecules 2009, 14 3925 by squalene synthase (SQS). Squalene is then oxidized biosynthetically by squalene epoxidase (SQE) to generate 2,3-oxidosqualene. Oxidosqualene cyclases (OSCs) ...
Keywords: ursolic acid; anticancer; triterpene; anti-inflammatory; antibacterial 1. Introduction Ursol1i.cInatrcoidduc(t3ioβn-hydroxy-urs-12-ene-28-oic acid, UA, Figure 1) is a pentacyclic terpenoid exhibiting a wUidrseolircaancigde(3oβ-fhpydhraorxmy-uarcse-1u2t-eicnae-l28p-rooicpa...