In this method, an active intein intermediate (specie 1) is mutagenized to replace the catalytic asparagine (step A) with a non-catalytic amino acid (Y) and to introduce a DNA-binding domain downstream of IN (step B) to yield specie 2. The intein-mediated cyclization reaction will proceed...
Its lower part (Triassic–Jurassic–Lower Cretaceous) presents striking similarities with the inter- basinal rises of the Helvetic realm (e.g. Internal Mont- Blanc), while its upper part (Upper Cretaceous to Tertiary) typically belongs to the North-Penninic (Valais s.l.) domain. Thus the ...