Both RmAb158 and its bispecific variant RmAb158-scFv8D3 achieved positive effects of long-term treatment. Despite its ability to efficiently enter the brain, the benefit of using the bispecific antibody in chronic treatment was limited by its reduced plasma exposure, which may be a result of ...
We further evaluated the ability of NNJA peptides on phage to deliver functional groups into HEK-239 and CaCo2 cells by displaying an Avi-tag peptide on the minor coat protein pIX. Post biotinylation of Avi-tag by BirA, streptavidin-HRP was added to phage, forming a phage-protein complex ...
The ability of the mentioned recombinant proteins to degrade Aβ was first evaluated in vitro using synthetic Aβ peptides followed by sandwich ELISA. For the in vivo studies, a single injection of 125-iodine-labelled sNEP-scFc-scFv8D3 and muNEP-scFc-scFv8D3 was intravenously administered to...
Antiplasmodial and prehemolytic activities of alpha-peptide-beta-peptoid chimeras ChemBioChem., 8 (2007), pp. 1781-1784 Google Scholar [29] J. Fernandez-Carneado, M.J. Kogan, S. Castel, E. Giralt Potential peptide carriers: amphipathic proline-rich peptides derived from the N-terminal doma...
In certain embodiments, further provided herein is a method of making a cell/tissue-specific cell-penetrating antibody, comprising: introducing into a host cell a vector comprising a polynucleotide encoding a VL with reduced of abolished HSPG binding ability, a polynucleotide encoding a VH, and a ...
Mac-1 alpha, Mac-1 beta, MFGE8, SLIT2, STX3, TCRA, TCRB, TCRD, TCRG, VTI1A, VTI1B, and any combinations thereof, but numerous other polypeptides capable of transporting a polypeptide construct to an EV are comprised within the scope of the present invention. The EV proteins as per ...
FIG. 2ashows the results of confocal microscopy observation of the cytosol-penetrating ability of the cytosol-penetrating antibody TMab4 or the cell-penetrating peptide TAT in the presence or absence of an inhibitor thereof. FIG. 2bis a bar graph showing the results of quantifying the FITC (gree...
The présent invention relates to cell-penetrating peptides characterized in that it comprises an amino acid séquence selected in the group consisting of X1LX2RALWX9LX3X9X4LWX9LX5X6X7X8, X1LX2LARWX9LX3X9X4LWX9LX5X6X7X8 and X1LX2ARLWX9LX3X9X4LWX9LX5X6X7X8, wherein X1 is beta-A or S,...
HSC transplantation; HU: Hydroxyurea; iPSCs: induced Pluripotent Stem Cells; PEG: polyethylene glycol; PRT: protein replacement therapy; PTD: protein transduction domain; PTs: proteins therapeutics; R: arginine; RBC: red blood cell; SCD: sickle cell disease; α:alpha; β: beta; γ:gamma, δ...
The surface of the CS/KH550/MAG hybrid membrane appeared as clear layered stripes because of the neater layers in the CS/KH550/MAG hybrid membrane and the coupling force of the interpenetrating layered structure made the self-supporting ability stronger. Therefore, the “interpenetrating petals” ...