All fluorescently labeled samples were visualized using a Nikon Alsi confocal microscope. Immunofluorescence staining for αSA on days 10, 20, and 30 cardiac tissues revealed sarcomere development over time with better defined and aligned sarcomeres by day 30 of differentiation (FIG. 2d). Results ...
The FITC-labeled MNPSNPs were detected at an excitation (Ex) of 488 nm and emission (Em) of 520 nm, while the nucleus (DAPI) was detected at Ex 405 nm, Em 470 nm and the cytoskeleton (phalloidin) was detected at Ex 570 nm, Em 603 nm. The images were then analyzed using the ...