9、纯病毒1、用超声波或冻融的方法使细胞破碎释放出病毒;2、 慢慢搅动并加入 NaCl终浓度是0.5mol/L,有助于病毒的沉淀,再等量加入10%PEG(一般使用的是PEG6000就可以了。如果对样品的纯度不是严格要求的话,可以建议使用Millpore 公司的离心浓缩柱,Amicon系列的可以一次浓缩15ml样品。);3、4 C过夜或放置一段时间...
Phase equilibria for the NaCl/KCl-PEG400-H_2 O aqueous ternary systems at 298 K were studied using an isothermal dissolution method. In addition,the densities and refractive indices were determined. The corresponding phase diagrams,as well as the density vs. composition and refractive index vs....
Placental explants were digested with 0.3 M NaOH or homogenization in an ice-cold 1.15% KCl solution. Total protein (mg) per explant was quantified using a Bio-Rad protein assay (Bio-Rad Laboratories, UK) according to the manufacturer’s instructions and compared to a standard curve prepared ...
KCl: Potassium chloride MgCl2·6H2O: Magnesium chloride hexahydrate Na2SO4 : Sodium sulfate NaCl: Sodium chloride NaHCO3 : Sodium hydrogen carbonate PEG: Polyethylene glycol, PVA: Polyvinyl alcohol SBF: Simulated body fluid SEM: Scanning electron microscopy TRIS: Trishydroxymethyl aminometha...
因此,稳定剂种类的选择至关重要,渗透压稳定剂主要是应用无机盐类(如NaCl、KCl、MgCl)和常见的有机物(如蔗糖、甘露醇、山梨醇)。丝状真菌原生质体的制备通常选择无机盐作为渗透压稳定剂,因此,本研究选用KCl作为稳渗剂,但是在浓度为0.8 mol/L时无法产生原生质体,后来,分别筛选了0.2 mol/L、0.4 mol/L、0.8 mol/...
PEG沉淀法提纯病毒1、用超声波或冻融的方法使细胞破碎释放出病毒;2、慢慢搅动并加入NaCl终浓度是0.5mol/L,有助于病毒的沉淀,再等量加入10%PEG(一般使用的是PEG6000就可以了。如果对样品的纯度不是严格要求的话,可以建议使用Millpore公司的离心浓缩柱,Amicon系列的可以一次浓缩15ml样品。);3、4℃过夜或放置一段...
PEG solution (40%, v/v) 1 g PEG4000 (Fluka, #81240) **Very Important!! 0.75 ml H 2 O 0.625 ml 0.8 M mannitol 0.25 ml 1M CaCl2 W 5 1000 ml 154 mM NaCl 9.0 g 125 mM CaCl 2 18.4 g 5 mM KCl 0.37 g 5 mM glucose 0.9 g 0.03% MES 0.3 g pH to 5.8 wi...
The chemical name is NaCl. The average Molecular Weight: 58.44. The structural formula is: Na+Cl- Potassium Chloride, USP The chemical name is KCl. The average Molecular Weight: 74.55. The structural formula is: K-Cl 12 CLINICAL PHARMACOLOGY ...
PEG solution (40%, v/v) 1 gPEG4000 (Fluka, #81240)**Very Important!! 0.75 ml H2O 0.625 ml0.8 Mmannitol 0.25 ml1MCaCl2 W 5 1000 ml 154 mMNaCl9.0 g 125 mMCaCl218.4 g 5 mMKCl0.37 g 5 mMglucose0.9 g 0.03% MES0.3 g pH to 5.8 with KOH autoclave 20 minutes in 125 bottles M...
0.75 ml H2O 0.625 ml 0.8 M mannitol 0.25 ml 1M CaCl2 W 5 1000 ml 154 mM NaCl 9.0 g 125 mM CaCl2 18.4 g 5 mM KCl 0.37 g 5 mM glucose 0.9 g 0.03% MES 0.3 g pH to 5.8 with KOH autoclave 20 minutes in 125 bottles MaMg solution 100 ml 15 mM MgCl2 1.5 ml 1M MgCl2 0.1% ...