The first approved PEGylated proteins were heterogeneous mixtures of conjugate isomers (e.g., PEG–asparaginase and PEG-adenosine deaminase), differing both in the number of coupled polymer chains and in the linkage sites. Recently, the requirements for approval have increased so that only thoroughly...
Effects of site-directed PEGylation on L-asparaginase thermostability Cunha, Jheniffer Rabelo Published date: 11 Aug 2021, https://doi.org/10.11606/D.9.2021.tde-05082021-101113 The impact of locally-delivered tacrolimus-releasing microspheres and polyethylene glycol-based islet surface modification on ...
e19504 Background: Nasal-type extranodal NK/T-cell lymphoma (ENKL) is a highly invasive disease with quite poor prognosis.Pegaspargase (PEG-Asp) displays a similar anti-cancer mechanism as that of L-asparaginase (L-ASP), but exhibits lig... Jing-Yun Wen,M Li,X Li,... - 《Journal ...
Fig. 3: Point mutations to residues of the ring-forming motif abolish antigen binding. Full size image Binding mechanism involves paratope conformational change Fig. 4: Anti-PEG Fab undergoes conformational change at binding site to create final open ring structure after initial interactions with PEG...
Abuchowski and co-workers were the first to develop a method for the reduction of the immunogenicity of proteins by their covalent attachment to methoxy poly(ethylene glycol) (PEG). They prepared PEG–catalase [11], PEG–trypsin [12], PEG–asparaginase [13], PEG–adenosine deaminase [14] an...
Glioblastoma multiforme (GBM) remains a cancer with a poor prognosis and few effective therapeutic options. Successful medical management of GBM is limited by the restricted access of drugs to the central nervous system (CNS) caused by the blood brain ba
of asparaginasebyconjugation to linearand branched monornethoxypol“ethyleneglyc01).J ControlRelease 1996;40:199-209 【i21 Hubcr R Kukle D,Rode D,Schwager只BaltelsK,Deisenhofer J,Steigemann LStructureofthe complex formed by bovine trypsin ...
They also hypothesized the mechanism of action for the three catalysts: DBU and TBD can activate the hydroxyl group of the initiator by hydrogen bonding and thus promoting the nucleophilic attack to the phosphorus centre of the monomer. TU, in contraat, is able to activate the PO-bond in ...