27.实施方案26的方法,其中所述UF/DF#3步骤(a3)进行所用的UF/DF#3膜的截留分子量(MWCO)为大约3kDa到大约20kDa。 28.实施方案27的方法,其中所述UF/DF#3膜的MWCO为大约8kDa到大约15kDa。 29.实施方案28的方法,其中所述UF/DF#3膜的MWCO为大约10kDa到大约12kDa。 30.实施方案29的方法,其中所述UF/DF#3...
4arm-PEG-NH2, MW 20kDa – 5 gram Item# 4arm-PEG-NH2-20K-5g $360.00 上海金畔生物科技有限公司提供生命科学研究领域系列产品,包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;修饰性PEG(Laysan bio、NANOCS、Avanti等品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、...
Sigma 56 months ago Unranked Hero Ranking 1 - 2 Record 4.50 KDA Ratio 33.33% Win Rate 13 minutes Time Played 20.21/ 10min Eliminations 13.47/ 10min Objective Kills 03:23/ 10min Objective Time 8,595/ 10min Damage 4.49/ 10min Deaths — Objective Contest Time — Weapon Accuracy 3.74/ 10...
实施例6:n端定点修饰的聚乙二醇化门冬酰胺酶的稳定性研究将培门冬酶、oncaspar(购自sigmatau制药公司)以及y-pald-40k-asp(di)分别用tris-hcl(9.0)的缓冲液稀释到1mg/ml。放入55℃水浴锅中,分别于0h、1h、2h、3h、4h、5h之后各取出100ul,用于酶活性检测。活性测定结果如图7所示。由图可以看出,经过水浴处理1...
Bovine spleen adenosine deaminase and 10-kDa PEG-diol were obtained from Sigma (St Louis, Mo). Analytical methods Testing for anti-PEG antibody A protocol for studying anti-PEG antibodies in stored, coded, and deidentified samples from RADAR participants was granted an exemption from review by ...
1.N端定点修饰的聚乙二醇化门冬酰胺酶注射剂,由N 端定点修饰的聚乙二醇化门冬酰胺酶、药用辅料和水组成,所述N 端定点修饰的聚乙二醇化门冬酰胺酶为聚乙二醇在L-门冬酰胺酶的2 个亚基的N 端伯氨基上偶联,所述聚乙二醇分子量为30 kDa 40kDa,聚乙二醇为分支型、活化基团为醛基;所述注射剂中N 端定点修饰的聚乙...
Paraformaldehyde, 2-methoxyethylamine and NMP were purchased from Sigma-Aldrich and used as received unless otherwise specified. The d6-DMSO was purchased from Cambridge Isotope Labs and used as received unless otherwise specified. Poly(ethylene glycol) diol 4.6 kDa and 8.0 kDa were end-funct...
PEG−amide-tetranorbornene (PEGaNB) was synthesized via amidification of PEG−tetra-NH2(20 kDa; JenKem USA) with 5-norbornene−2-carboxylic acid (Sigma-Aldrich)2. Briefly, anhydrous dichloromethane (DCM; Sigma-Aldrich), 5 molar equivalents of N,N’-diisopropylcarbodiimide (DIC; Sigma-Al...
poss-peg杂化水凝胶通过以下方法来制备:将4-arm-peg-mal(北京健凯科技有限公司)和poss-sh(sigma公司)溶解于3ml四氢呋喃中,室温搅拌3.5小时后除去溶剂得poss-peg预聚物;然后将poss-peg预聚物与基质金属蛋白酶底物肽(上海吉尔生化科技公司)溶解于3mlph7.4的三乙醇胺中,混合均匀,得预成胶溶液;最后将上述预成胶溶液...
预制的10% tris-甘氨酸凝胶购自NOVEX(SanDiego,CA,USA)。蓄水池(reservoir)体积200μl、泵速度1.0μl/hr以及维持时间(duration)7天(模式2001)的微型渗透泵,购自DURECT CORPORATION(Cupertino,CA,USA)。PLP和其他试剂购自SIGMA(St.Louis,MO,USA)。 活化的PEG衍生物以PEG比rMETase中游离的赖氨酸(每rMETase分子32...