pEASY®-T1 Cloning Vector包含LacZ基因,在含有IPTG和X-gal的平板培养基上,可进行蓝白斑筛选,适用于TA克隆。 产品组成 References 1 Lu S, Luo S, Liu C, et al. Induction of significant neutralizing antibodies against SARS-CoV-2 by a highly attenuated pangolin coronavirus variant with a 104nt deleti...
3端悬垂胸腺嘧啶t可用于ta克隆拓扑异构酶与载体相互偶联活化载体taqdna聚合酶具有类似脱氧核糖核酸末端转移酶tdt的功能可在新合成双链产物的3端加上一个碱基 pEASY 目录号: 试剂盒组成 1.pEASY- T1 Cloning Vector (10 ng/μl) 2.Control Template (5ng/μl),Control Primers (10 μM), 3.M13F (10 μ...
pEASY®-T1 Simple Cloning Vector消除了pEASY®-T1 Cloning Vector上的多克隆位点,克隆后的PCR产物无法使用pEASY®-T1Cloning Vector上多克隆位点的限制内切酶切下,包含LacZ基因,在含有IPTG和X-gal的平板培养基上,可进行蓝白斑筛选,适用于TA克隆。
pEASY®-T1 Cloning Kit 目录号: CT101 试剂盒组成 1. pEASY- T1 Cloning Vector (10 ng/μl) 2. Control Template (5ng/μl), Control Primers (10 μM), 3. M13F (10 μM), M13R (10 μM) 4. Trans1-T1 Phage Resistant Chemically Competent Cell. 保存 Trans1-T1 Phage Resistant Chemica...
Competent Cell at -70°C for six months; others at -20°C for 9 months Shipping Dry ice (-70℃) Product Contents ComponentCT101-01 (20 rxns)CT101-02 (60 rxns) pEASY®-T1 Cloning Vector (10 ng/μl)20 μl3×20 μl Control Template (5 ng/μl)5 μl5 μl ...
1.pEASY- T1 Cloning Vector (10 ng/μl)2.Control Template (5ng/μl), Control Primers (10 μM),3.M13F (10 μM)，M13R (10 μM)4.Trans1-T1 Phage Resistant Chemically Competent Cell.保存 Trans1-T1 Phage Resistant Chemically Competent cell 可于–70℃保存至少六个月, 其他组分-20℃保存...
1.pEASY-T1CloningVector(10ng/μl) 2.ControlTemplate(5ng/μl),ControlPrimers(10μM), 3.M13F(10μM),M13R(10μM) 4.Trans1-T1PhageResistantChemicallyCompetentCell. 保存 Trans1-T1PhageResistantChemicallyCompetentcell–可于70℃保存至少六个月,组组其他分- ...
隆载体(例如pEASY—Bluntcloningvector) 等¨都可采用本研究中的方法,加入多克隆位 点,进行多DNA片段的连接克隆.因此,预计 本方法的应用前景将非常广阔. 参考文献: [1]BolivarF,RodriguezRL,GreenePJ,eta1.Construc— tionandcharacterizationofnewcloningvehicles.II.A multipurposecloningsystem[J].Gene,1977,2(2)...
1.pEASY-T1CloningVector(10ng/μl) 2.ControlTemplate(5ng/μl),ControlPrimers(10μM), 3.M13F(10μM),M13R(10μM) 4.Trans1-T1PhageResistantChemicallyCompetentCell. 保存 Trans1-T1PhageResistantChemicallyCompetentcell可于–70℃保存至少六个月,其他组分- ...
(-20) priming site: bases 388-403 f1 origin: bases 545-982 Kanamycin resistance ORF: bases 1316-2110 Ampicillin resistance ORF: bases 2128-2988 pUC origin: bases 3133-3806 pEASY-T1 vector Restriction Map Enzyme # of cuts positions Acc65I (1) 246 AceIII (3) cagctc 948, 2629, 3869 ...