1. 观察两个单染管,发现 PE 单染管出现假阳性,需要补偿,下面进行自动补偿。 2. 选择空白管和两个单染管,拖进「Compensation」,然后点击类似于小山的图标。 3. 仪器默认会出现很多通道,可以删除自己不需要的。例如本次分析中只需要 7-AAD 和 PE,其余可以删除...
1、选择仪器的补偿界面( compensation ),选择有补偿的两个检测通道来调节,如果不确定如何调节(两个通道之间互减),可试试两种减法都试试,看是那种减法趋向正确的细胞分布。一般来说漏到哪个通道,哪个通道就是被项,例如FITC漏到了PE,那么就是PE来减去FITC; 2、荧光溢漏的所有染料都需要设置补偿单染管,每个单染管...
FITC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC PE Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC APC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC APC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC PE Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC ...
Compensation Beads FC Labeling Kits FC Catalog # 11-1661-82 Invitrogen CD166 (ALCAM) Monoclonal Antibody (eBioALC48), FITC, eBioscience™ CNY 2,337.00 Unit Size : 100 µg 添加到购物车 Catalog # 12-1661-82 Invitrogen CD166 (ALCAM) Monoclonal Antibody (eBioALC48), PE, e...
TIGIT Monoclonal Antibody (MBSA43), FITC, eBioscience™ Price (CNY) 3,999.00 Unit Size : 100 Tests 添加到购物车 Catalog # 12-9500-42 Invitrogen TIGIT Monoclonal Antibody (MBSA43), PE, eBioscience™ Price (CNY) 2,948.00 飞享价 Ends: 31-Dec-2025 3,948.00 Save 1,000.00 ...
的荧光信号是由一定比例的FITC荧光信号和PE荧光信号共同组成,流式细胞术用补偿调节的方法实现这个目的:排除FL2中来自于FITC的荧光信号,就是设置“FL2-FL1”(“PE-FITC”)的补偿值,使FL2完全代表PE的荧光信号:排除FL1中来自于PE的荧光信号,即设置“FL1-FL2”(“FITC-PE...
Compensation of spectral overlap for FITC and PE.Christina, YipVera, IgnjatovicChantal, AttardPaul, MonagleMatthew, D. Linden
Regulatory Status RUO Other Names Ly77, T and B cell activation marker Isotype Rat IgM, κ Ave. Rating Submit a Review Product Citations 6publications BALB/c mouse bone marrow cells were stained with anti-mouse IgD FITC and anti-mouse GL7 (clone GL7) PE/Cyanine7 (lef...
(100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can ...
The following controls are used to set up compensation and quadrants: 1. Unstained cells. 2. Cells stained with PE Annexin V (no 7-AAD). 3. Cells stained with 7-AAD (no PE Annexin V). Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used...