In this study, we aimed to characterize the role of peroxisomes in mitochondrial health and stress-induced regulatory responses. We generated a stable zebrafish model of ZSD by targeting thepex5gene. Zebrafish with homozygouspex5mutations (pex5−/−) share critical features of ZSD in humans.pe...
After treating with TEV protease for 18 h at 18 ◦C, a quarter of the MBP fusion protein was cleaved by the TEV protease (data not shown). The mixture was then dialyzed against buffer B and passed through an amylose column. On the amylose column, cleaved HER2(scFv)-GFP and TEV ...