The pdgfra-H2BGFP reporter mouse visualizes expression of Pdgfra by expression of GFP bound to histones. These leads to a strong stabilization of the GFP molecule, leading to a) a strong nuclear signal and b) retention of fluorescence even after the cells no longer express Pdgfra. In the ...
d, (Left) Fluorescent images of Tppp3CG/+;R26RtdT;PdgfraH2B-eGFP Achilles or tail tendon 5 d after TMX-induced marking; n=6-9 animals; dashed line, midsubstance-sheath boundary; arrowheads same code as b; direct fluorescent imaging to visualize GFP signal from PdgfraH2B-eGFP. (Right) ...