重组抗PD-1全人单抗体(GMP级)价格:￥1400/瓶产品详情:重组抗PD-1全人单*抗体(GMP级)Recombinant anti-Human PD-1 Functional Monoclonal Antibody产品说明:PD1全人单抗可以有效地封... 纯度:98% 包装规格:1mg 用途:分析、研发、生产 货号:kx-GMP-001 ...
次日,添加25μl/孔PD1样品或人抗PD1(Roche)/小鼠抗PD1(Biolegend;目录:329912)参照抗体并于4℃温育2小时(以避免内在化)。小心清洗(1x90μl/孔PBST)后,通过添加30μl/孔在1xPBS缓冲液中稀释的0.05%戊二醛(Sigma,目录号:G5882,25%)并于室温温育10分钟来固定细胞。清洗(3x90μl/孔PBST)后,添加25μl/孔...
an Antibody byBioLegend Applications: FC/FACS Reactivity: Mus musculus (House mouse) (124610)PE anti-mouse CD40 Antibody an Antibody byBioLegend Applications: FC/FACS Reactivity: Mus musculus (House mouse) (135220)Brilliant Violet 605™ anti-mouse CD279 (PD-1) Antibody ...
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通过用SA‑APC(博奇公司(Biolegend))染色确定TIGIT受体的 表面表达。通过流式细胞术确定MFI。所有数据点一式两份。结果以平均值±SD表示。 [0046]图9A显示抗TIGIT mAb对人外周血单核细胞(PBMC)的ADCC作用。通过流式细胞术 ++‑+ 确定健康供体的PHA刺激的PBMC上的TIGIT表达。CD4(CD4Foxp3)、CD8T效应子和...
(in house production), anti-rabbit Alexa Fluor 647 (secondary antibody for TAG, ab150075), F4/80-PE (eBioscience, clone BM8), MHCII-FITC (Biolegend, clone M5/114.15.2), Mrc1-AF647 (Biolegend, clone C068C2), High Endothelial Venule -AF488 (eBioscience, clone MECA-79) and counter...
Subsequently, the single-cell suspensions obtained were pre-incubated with a monoclonal antibody targeting mouse CD16/CD32 (Fc block; 2.4G2; BD Pharmingen) and labeled with fluorochrome-conjugated antibodies targeting mouse CD45, CD8, PD-1, Tim3, CD44, and CD62L (all obtained from Biolegend...
Effect of YIV-906 on the anti-tumor of Anti-PD1 (YIV-906 500 mg/kg p.o. bid × 7, Anti-PD-1 antibody 200ug/mice i.p. once) against Hepa 1–6 tumor growth of C57BL6 mice. (A) Individual tumor growth (indicated as spot plot with different colors and symbols) of each tre...
(DMEM medium + P. anaerobiuswith antibiotics; Fig.2aand Extended Data Fig.4b). To evaluateP. anaerobius-induced secretary factors in CRC cells, we performed a chemokine antibody array of conditioned medium from CRC cells with or withoutP. anaerobiustreatment (Fig.2b).P. anaerobius...
A combined treatment strategy using IFNγ and anti-PD-1 antibody significantly increased T cell killing of tumor cells in vitro and showed synergistic inhibition of tumor growth in a mouse model of CRC. CyTOF found drastic changes in the immune microenvironment upon combined immunotherapy. Treatment...