严格的病理诊断需要用到严格生产的检测试剂，达到了以上全部免疫组化检测条件，还需要选择一支优质的PD-L1抗体，方能让你的免疫组化结果更完美.ABclonal致力为生命科学工作者提供优质的服务于产品，生产包括病理诊断抗体和科研类抗体，严格把控各项病理诊断指标，部分PD-L1抗体经病理IHC验证及KO验证，应用种类多、特异性...
严格的病理诊断需要用到严格生产的检测试剂,达到了以上全部免疫组化检测条件,还需要选择一支优质的PD-L1抗体,方能让你的免疫组化结果更完美. ABclonal致力为生命科学工作者提供优质的服务于产品,生产包括病理诊断抗体和科研类抗体,严格把控各项病理诊断指标,部分PD-L1抗体经病理IHC验证及KO验证,应用种类多、特异性强、...
ABclonal现有波士顿抗体与蛋白研发中心、上海诊断与科研分子酶产品研发基地以及中国武汉光谷诊断与科研免疫产品生产和研发基地。经过多年的深耕与发展,ABclonal已拥有免疫与分子酶两大技术平台,集新型分子酶、全领域应用NGS建库解决方案、新平台单克隆抗体与高活性蛋白制品的研发与生产于一体。ABclonal致力成为产业科研转化的核...
ABclonal致力为生命科学工作者提供优质的服务与产品,生产包括病理诊断抗体和科研类抗体,严格把控各项病理诊断指标,部分PD-L1抗体经病理IHC验证及KO验证,应用种类多、特异性强、效价高,是广大免疫检查点领域研究者的最佳选择! PD-L1/CD274 Rabbit mAb (A2034...
(IFN-γ,Peproted公司);IFN-γ稀释液(杭州联科生物技术有限公司);流式细胞术PD-L1抗体(eBioscience公司);免疫荧光PD-L1抗体、GAPDH抗体(Proteintech公司);哺乳动物雷帕霉素靶蛋白(mTOR)抗体(Abclonal公司);蛋白激酶B(Akt)、p-Akt、PI3...
purchased from Cell Signaling Technology (Boston, MA, USA) and Abcam (Cambridge, CB2 0AX, UK), respectively. The IgG isotype control was purchased from Cell Signaling Technology (Boston, MA, USA). A secondary antibody specific to the IgG light chain was purchased from ABclonal (Wuhan, China...
Then membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (ABclonal) at room temperature for 1 h after being washed by TBST. Immunoreactions were visualized using a NcmECL Ultra kit (NCM Biotech#P10300). Image acquisition and quantitation of intensity were performed ...
对于自然生命来说,衰老似乎是不可避免的,随着年龄的增长,衰老细胞会在各种组织器官中积累,诱发过度炎症,从而导致组织内稳态失衡和机体的衰败。 该研究发现,PD-L1在衰老细胞中异质性表达,PD-L1阳性衰老细胞会随着年龄的增长而不断在体内积累。通过PD-1/PD-L1免疫检查点阻断疗法来清除PD-L1阳性的衰老细胞,或将成为...
[0088]6)一抗杂交:将封闭后的PVDF膜浸入一抗(PD‑L1或GAPDH,GAPDH为内参蛋白,PD‑L1购买自ABclonal,货号为A1645)工作液(用5%BSA 1:1000配制)中,4℃孵育12小时,取出PVDF膜,在TBST中清洗3次,每次5min; [0089](7)二抗孵育:将PVDF膜浸入酶标二抗(Anti‑rabbit IgG,HRP‑linked Antibody)溶液(用5%BSA ...
ABclonal (Wuhan, China). Anti-PD-L1 was purchased from BioXcell (USA). Tumor necrosis factor alpha, interferon gamma and interleukin 6 ELISA kit were purchased from Uscn Life Science, Inc., (China). Anti-CD3+-FITC, anti-CD8+a-APC and anti-CD4+-PC5.5 were purchased from Biolegend (...