MEDI5752介导了PD-1的内化和降解 有报道显示，细胞膜表面80%的CTLA-4会在5分钟内被快速内化（internalization），因此在任意时间点，细胞膜表面都只会有少量的CTLA-4存在。研究人员探究了MEDI575及其亲本抗体在PD-1+ CTLA-4+ CHO细胞、人原代CD4+ 和CD8+ T细胞中的内化能力。结果显示MEDI5752和只靶向CTLA-4的抗...
MEDI5752介导了PD-1的内化和降解 有报道显示,细胞膜表面80%的CTLA-4会在5分钟内被快速内化(internalization),因此在任意时间点,细胞膜表面都只会有少量的CTLA-4存在。研究人员探究了MEDI575及其亲本抗体在PD-1+CTLA-4+CHO细胞、人原代CD4+和CD8+T细胞中的内化...
有报道显示,细胞膜表面80%的CTLA-4会在5分钟内被快速内化(internalization),因此在任意时间点,细胞膜表面都只会有少量的CTLA-4存在。研究人员探究了MEDI575及其亲本抗体在PD-1+ CTLA-4+ CHO细胞、人原代CD4+ 和CD8+ T细胞中的内化能力。结果显示MEDI5752和只靶向CTLA-4的抗体(anti-CTLA4 mAb和mvCTLA4)都能被...
有报道显示,细胞膜表面80%的CTLA-4会在5分钟内被快速内化(internalization),因此在任意时间点,细胞膜表面都只会有少量的CTLA-4存在。研究人员探究了MEDI575及其亲本抗体在PD-1+ CTLA-4+ CHO细胞、人原代CD4+ 和CD8+ T细胞中的内化能力。结果显示MEDI5752和只靶向CTLA-4的抗体(anti-CTLA4 mAb和mvCTLA4)都能被...
PD-1/PD-L1-IN-29 (S4-1) is a potent PD-1/PD-L1 inhibitor with an IC 50 value of 6.1 nM. PD-1/PD-L1-IN-29 binds PD-L1 and disrupts PD-1/PD-L1 interactions, induces PD-L1 dimerization and internalization, improves its localization to the endoplasmic reticulum, and promotes PD-L1...
Nivolumab outperforms pembrolizumab in human Tcells, while PD-1 internalization requires crosslinking by bivalent antibody. While mono- and bivalent anti-PD-1 inhibit tumor growth with CD8 tumor-infiltrating cells expressing increased granzyme B, bivalent antibody is more effective where the combination...
[4]Lim S H, Vaughan A T, Ashton-Key M, et al. Fc gamma receptor IIb on target B cells promotes rituximab internalization and reduces clinical efficacy[J]. Blood, 2011, 118(9): 2530-2540. 本文作者丨谭硕
Fig. 4: Exo-PD-1 induces cell surface PD-L1 internalization. A Immunoblot of cellular PD-1 and exosome uptake in BT549-PD-L1 cells with Exo-Con or Exo-PD-1 treatment. B Immunoblot of cellular and exosomal PD-1-EGFP expression in doxycycline-inducible Jurkat-PD-1-EGFP cell line. C Co...
[4]Lim S H, Vaughan A T, Ashton-Key M, et al. Fc gamma receptor IIb on target B cells promotes rituximab internalization and reduces clinical efficacy[J]. Blood, 2011, 118(9): 2530-2540.
初步认为Exo-PD-1可以恢复PD-L1对T细胞抑制功能,总的来说可以阻断肿瘤PD-L1和T细胞PD-1的结合。 Exo-PD-1 induces PD-L1 internalization via clathrin-mediated endocytosis Exo-PD-1通过网格蛋白介导内吞作用诱导PD-L1内化: 1、细胞-外泌体孵化WB结果显示Exo-PD-1对应的PD-1表达量更多,表明Exo-PD-1比Exo-...