该试验由两种基因工程细胞系:PD-1效应细胞(PD-1 Effector Cells),即稳定表达人PD-1受体和转录因子诱导的荧光素酶报告基因的Jurkat T细胞;PD-L1 aAPC/CHO-K1细胞(aAPC PDL1 CHO Cell Line),是一种稳定表达人PD-L1和一种以抗原非依赖方式激活同源TCR的细胞表面蛋白的CHO-K1细胞。 当两种细胞共培养时,PD-1/...
PD-1 is a co-inhibitory receptor mainly expressed on the surface of T cells [20]. PD-1’s primary function is to suppress the T cells’ activity by the regulation of the TCR signaling cascade [21,22,23]. High PD-L1 expression in tumor microenvironment is frequently observed in many typ...
Kaempferol —— The blockade of PD-1/PD-L1 interaction PD-1 /PD-L1 binding affinity and competitive inhibition Human PD-1 Jurkat cells and PD-L1/aAPC CHO-K1 cells [125] Non-flavonoid polyphenols Curcumin Head and neck cancer Synergistic effect with anti-PD-1 Ab, ↓PD-L1 expression Reinvig...
Gem vs ATG + Gem:P = 0.04) over total live cells in Pan02 tumors from each treatment group, measured by flow cytometry. *P < 0.05, **P < 0.01, One-way ANOVA with Tukey’s multiple comparisons. Average percentages of PD-L1 expression ongCD4+T cells (Con vs ATG ...
pd-1是i型跨膜蛋白,主要表达在活化的b细胞、t细胞和骨髓细胞(chen等人2013,nat.rev.immunol.13:227-42),有两种细胞表面的糖蛋白配体,分别是pd-l1和pd-l2。pd-1与pd-l1或pd-l2结合后,会下调t细胞的功能,包括降低t细胞的活化、分化增殖和细胞因子的分泌等。阻断pd-1和pd-l1的相互作用能够逆转免疫抑制,而...
Western blot analysis confirmed that aAPCs express human PD-L1, while original CHO-K1 cells and CHO-K1 cells transfected with the TCR Activator-coding vector but not the human PD-L1-encoding vector did not show any expression of this protein (Figure 5 A). Moreover, the human PD-L1 ...
CHO-K1 model cells (hPD-L1 aAPCs) are suitable for testing PD-L1-binding compounds, we have verified the expression of human PD-L1 protein in these cells. Western blot analysis confirmed that aAPCs express human PD-L1, while original CHO-K1 cells and CHO-K1 cells transfected with the TCR...