PD-10DesaltingColumnsareusedfordesalting,bufferexchangeand samplecleanup.Smallmoleculeslikesalt,freelabelsandother impuritiesareefficientlyseparatedfromthehighmolecularweight substancesofinterest. Thechromatographytechniqueisgelfiltrationandmoleculesare separatedonthebasisofdifferencesinsize. p.3 ...
PD-10 Desalting Columns are used for desalting, buffer exchange and sample clean up. Small molecules like salt, free labels and other impurities are efficiently separated from the high molecular weight substances of interest.The chromatography technique is gel filtration and molecules are separated on ...
PD-10 Desalting Columns are prepacked and designed for rapid, convenient sample clean-up of proteins and other large biomolecules (>5000 M r).PD-10 Desalting Columns can be used in a wide range of applications such as desalting, buffer exchange and removal of low-molecular weight compounds.
and PD MultiTrap G-25PD-10 Desalting Columns, PD MidiTrap™ G-25, PD MiniTrap™ G-25, PD SpinTrap™ G-25, and PD MultiTrap™ G-25 (Fig 1) are prepacked, single-use columns and 96-well filter plates for buffer exchange and cleanup of biological samples, for example proteins and...
The PD-L1 IgV domain/KN035 complexes were subsequently purified using ion-exchange and gel filtration columns (GE Healthcare). Crystallization of PD-L1 and its complex with KN035 Both purified PD-L1 and PD-L1/KN035 complexes were concentrated to ~15 mg ml−1 and screened for ...
three columns (TSK gel, Super HM-M, Super HM-N and Super H3000), and THF was used as an eluent at 40 1C The GPC was calibrated with standard polystyrene samples. Elemental analyses were carried out using a Thermo- Finnigan Flash EA1112 CHN-O analyzer (Thermo-Finnigan, Milan, Italy)....
Gel filtered proteins were labeled with either SNAP-Cell 505 (NEB, S9103S), SNAP-Cell TMR (NEB, S9105S) or SNAP-Cell 647-SiR (NEB, S9102S) following manufacturer’s instructions. Free dyes were then removed using Zeba Spin Desalting Columns (Thermo Fisher Scientific, P187769). All ...
EVs were isolated by size exclusion chromatography (SEC) using Izon qEVoriginal/70 nm columns (IZON, SP1) as follows: On day 1, cells were seeded in 150 mm dishes. For cells growing in media supplemented with FBS, the culture medium was exchanged with fresh medium supplemented with 10% ult...
Molecular weights and distributions were estimated by size-exclusion chromatography on a Jasco Gulliver system (JASCO, Tokyo, Japan) equipped with two consecutive linear polystyrene gel columns (Tosoh TSK-gel GMHXL and G5000HXL) at 30 1C (column flow rate of 0.85 ml minÀ1) according to ...
modified anti-CD3 antibody, the same modified anti-CD105 antibody, the same modified anti-isolectin B4 antibody, and anti-FITC bead. These vascular endothelial cells were purified by positive selection using Magnetic-activated cell-sorting separation columns (the trade name: purchased from Miltenyi ...