PCR-Targeting操作方法经验总结PCR-Targeting实验步骤 ---李舜莹 1.验证质粒,确认质粒是否正确 2.所需片段的准备 1.以pJTU2170为模板,以带XbaI,NheI位点的kana抗性基因表达引物为引物,用KOD酶P出所需模板1,跑胶回收既得 2.以模板1为模板,以PCR-Targeting引物为引物,用rTaq酶P出所需片段,跑胶回收既得 3....
LMJS培训-链霉菌遗传操作和PCR-TARGETING-Sep2011 GeneticManipulationofStreptomycesandPCRTargeting MeifengTaoSep7,2011 Streptomycetesaremulticellularprokaryotes Streptomycescellsare multinuclear.http://openwetware.org/wiki/Streptomyces:Protocols GeneticmanipulationofStreptomyces CellWall E.coli-StreptomycesIntergeneric...
(感受态细胞浓度越高电转效果越好) 8. 在预冷的0.2cm的电转化杯中混合50ul感受态细胞与1-2ul PCR产物(经纯化,可尽量浓,但不能加得过多),混匀后立即电击,电击条件:200Ω,25uF,2.5kv, 电击结果为4.5~4.9ms 较理想。(电转化杯在70%乙醇中保存,用前先在无菌环境下用无水乙醇洗一下,再吹干,放冰上预冷,...
棒状链霉菌(Streptomyces clavuligerus)可产生多种β-内酰胺类化合物,其中包括克拉维酸.利用聚合酶链式反应(PCR)-Targeting体系构建棒状链霉菌突变株是获得克拉维酸高产菌株的又一可行方法.采用该体系对棒状链霉菌的lat基因进行了敲除,获得了没有抗性标记的lat基因被敲除的突变株S.clavuligerus lat::scar,其克拉维酸产量...
PCR-targeting Conjugative transfer and screening for double cross-overs FLP-mediated excision of disruption cassette λ RED Recomb. plasmid ts gam bet exo induction of λ RED by L-arabinose E.coli ET12567 / pUZ8002 / cosmid cosmid ...
10:24:05:PcR-Targeting是啥意思？干嘛用的，有人知道吗？谢谢 主要用于链霉菌中基因阻断用的！
Real-time PCR targetingOmpAgene for detection ofCronobacterspp. in powdered infant formulaCronobacter spp.real-time PCRTaqManCronobacter spp. (formerly Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is a rare cause of invasive infection with a high mortality rate...
Different PCR-based methods targeting microsatellites, kinetoplastic DNA (kDNA), telomeric sequences, or gp63, hsp70, miniexon, β-tubulin, and rRNA genes have already been proposed [5]. More recently, real-time PCR, a platform that can process a sample in less than an hour, has been ...
1) PCR Targeting System PCR定向突变系统 1. Development and Application of thePCR Targeting Systemin Actinobacillus Pleuropneumoniae & Serologic Detection of Actinobacillus Pleuropneumoniae in Swine by OmlA Enzyme-Linked Immunosorbent Assay; 胸膜肺炎放线杆菌PCR定向突变系统的初步建立及胸膜肺炎放线杆菌OmlA...
Linde.Rapid detection of Panton–Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene[J]. European Journal of Clinical Microbiology & Infectious Diseases .2007(2)Reischl U, Tuohy MJ, Hall GS, Procop GW, Lehn N, Linde H: Rapid detection of Panton-...