PBRT kit - PCR based replicon typing95°C for 10min 95°C for 60sec 60°C for 30sec 72°C for 60sec 72°C for 5min cool down to 4°CViale Piceno
Characterisations of plasmids were done using 5 different sets of Inc primers. The plasmids were also subjected to restriction digestion using different specific restriction enzymes.S. SonaM. AswathyBiya BellerminJaya Vinny EapenAnu Yamuna Joseph...
PCR-Based Replicon Typing (PBRT) for IncL and IncM plasmids: list of plasmids used for the specificity assay.Alessandra, CarattoliSalome, N. SeiffertSybille, SchwendenerVincent, PerretenAndrea, Endimiani
2005. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 63:219 -228. http://dx.doi.org/10.1016/j.mimet.2005.03.018.Carattoli A , Bertini A , Villa L , Falbo V , Hopkins KL & Threlfall EJ ( 2005 ) Identification of plasmids by PCR-based replicon typing ....
Eighteen pairs of primers were designed to perform 5 multiplex- and 3 simplex-PCRs, recognizing FIA, FIB, FIC, HI1, HI2, I1-Igamma, L/M, N, P, W, T, A/C, K, B/O, X, Y, F, and FIIA. The specificity of the method was tested on a collection of 61 reference plasmids ...
The PCR-Based Replicon Typing (PBRT), developed by Carattoli et al. in 2005, was an efficient method for plasmid identification and typing in Enterobacteriaceae. The 2005 PBRT scheme detected 18 replicons in 8 PCR reactions. Recently, the identification of novel replicons and plasmid types ...
Identification of plasmids by PCR based replicon typing in bacteremic Klebsiella pneumoniaeK. pneumoniaePlasmid typingIncCarbapenemaseColKP3Hybrid assemblyBackground: Klebsiella pneumoniae is a notorious pathogen with plasmid mediated resistance to all classes of antibiotics. It is important to determine the...
Onnberg, A., Soderquist, B., Persson, K., and Molling, P. (2014). Characterization of CTX-M-producing Escherichia coli by repetitive sequence-based PCR and real-time PCR-based replicon typing of CTX-M-15 plasmids. APMIS 122, 1136-1143. doi: 10.1111/apm.12270...
pneumoniae. Materials and methods Ninety consecutive K. pneumoniae collected over a period of three months from blood cultures were characterised by PCR for plasmid profile. Inc plasmid types were determined by PCR based replicon typing (PBRT) and carbapenemases were determined by multiplex PCR. For ...