Bands were visualized by UV illumination of ethidium-bromide-stained gels and captured using a Kodak Gel Logic 100 Imaging System and 1D Image Analysis Software (Eastman Kodak, Rochester, NY) and relative band intensity quantified using Photoshop Image Analysis tools by Adobe (San Jose, CA) for ...
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Quantitative real-time PCR for detection of OT-1 CD8+T cells diluted into normal C57Bl/6 splenocytes To test the sensitivity of the real-time assay, OT-1 CD8 +T cells were purified from an OT-1 transgenic mouse. OT-1 cells were diluted into splenocytes isolated from normal C57BL/...