Operation procedure is as following: concentrating objective bacterium (heat resisting bacterium), pre-amplifying bacterium, releasing nucleic acid from bacterium with wall broken, picking-up nucleic acid, and carrying out PCR amplification, gel electrophoresis test for amplified production, determining ...
Previously, amplification of DNA involved cloning the segments of interest into vectors for expression in bacteria, and took weeks. But now, with PCR done in test tubes, it takes only a few hours. PCR is highly efficient in that untold numbers of copies can be made of the DNA. Moreover,...
The differences between two groups were found to have statistical significance for detection of Aa(P 0.05). Conclusion The PCR can be used to detect Aa and observe the bacteria changes induced by wearing fixed orthodontic appliances. 展开 关键词: PCR Actinobacillus actinomycetemcomitans fixed ...
This universal PCR assay can help to demonstrate evidence of bacteria, but alone is usually insufficient for definitive identification and requires ancillary testing, such as species-specific hybridization probes or nucleic acid sequencing. We have developed an original real-time PCR assay that detects ...
The process is performed in a test tube and takes only a few hours. It's common to come across this term in chronic fatigue syndrome research that involves testing for pathogens, such as viruses or bacteria. When used in this way, PCR allows scientists to identify the source of the ...
Likewise, a person with an upset stomach and diarrhea could be tested for a range of bacteria, viruses and parasites that cause gastrointestinal infections, allowing a doctor to prescribe the appropriate regimen at the first visit instead of taking an empirical approach. ...
Keywords: Ralstonia pickettii; pharmaceutical water; PCR-nucleic acid test strip; drugs; contaminating bacteria 制药用水是药品生产中最重要的原料,制药用水的 质量直接影响药品的质量,尤其是灭菌的注射用水.灭 菌的注射用水常用于灭菌粉末的溶剂或注射剂的稀释 收稿日期:2022-06-08 基金项目:吉林省科技厅科技...
of Enterococcus bacteria culture method is better than the PCR test，but no significant data comparison（P > 0.05）；patient satisfaction with PCR test method is obviously better than that of bacterial culture method was statistically significant（P < 0.05）.Conclusion：The accuracy of PCR test ...
Broad-Range PCR for the Identification of Bacterial and Fungal Pathogens from Blood: A Sequencing ApproachSepsisBroad-range PCRSequencingSepsiTestIdentificationBacteriaFungiBroad-range PCR has become a valuable tool for the identification of microorganisms in the clinical laboratory over the last years. It...
quinolone resistance geneWe investigated the prevalence of qnr and aac(6')-Ib-cr genes in water-borne environmental bacteria and in clinical isolates of ... R Zhang,T Ichijo,YL Huang,... - 《Microbes & Environments》 被引量: 41发表: 2012年 New genetic environments of aac(6')-Ib-cr ge...