Clinical validation of the 3-codon assay in the LC480 instrument showed the assay detected 94% (81/86) of the specimens as WT or VOCs and 6% (5/86) of the tests producing indeterminate results compared to sequencing. Sanger sequencing also failed for four samples. None of the specimens ...
False-positive results can also occur where closely related species are highly similar to the target species leading to amplification. Fortunately, false-positive results are avoided in the multiplex assay described as species-specific amplicons are also size specific, so in cases of cross-...
Statistical differences in the virus V-qPCR activity (log viable viral particles mL−1) between the control sample and thermally treated samples for EhV, ASFV and PRSSV were evaluated by an ANOVA test (Tukey’s test) at the 95% confidence level (p < 0.05). EhV inactivation kinetics ...
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A common problem that results in spurious product formation is associated with the assembly of reactions at room temperature or on ice in a nonhot-start format: during the initial ramping (raising the reaction temperature) to 94°C needed to achieve initial melting and then to initiate the cycl...
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RESULTS SMN1 deletion test and SMN copy number analysis The SMN1 deletion test and SMN1 copy number analysis in 20 patients with SMA type I, SMA type III or SMA type IV revealed the absence of SMN1 exon 7 in all of these patients (Supplementary Table 2). The overall distribution of ...
To test the usefulness of our conjugation and immuno-PCR method for other (intracellular) proteins, we have performed similar antibody-dilution experiments using a wide variety of conjugates against > 40 (mostly intra- cellular) proteins (data not shown). The average squared correlation of these...
The results were analyzed using Microsoft® Office Excel 2007 and IBM SPSS® Statistics version 19.0. The Chi-square test was used to estimate the difference in AMR gene dissemination between the two farms, and P < 0.05 was used as the cutoff for significance. The Pearson Chi-square test...
knowlesi was 98.1% (95% CI 89.7–100), with 51 out of 52 samples recording positive results. The single false negative result was recorded from a 59-year old female patient with a parasitaemia of 12,968 parasites/µL. Specificity of the QuantiFast™ test was 98.8% (95% CI 93.4–...