The primer ratio (F3/B3: FIP/BIP: LF/LB) used for the assay was 1:8:2 with the final concentrations of 0.2 µM, 1.6 µM and 0.4 µM for F3/B3, FIP/BIP and LF/LB. LAMP reactions were performed and optimised in the Genie III triplicates and repeated three times. The assay...
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[44] was used to estimate data-set-specific parameters as nucleotide frequencies, the percentage of invariable sites, the transition/transversion ratio and the alpha-parameter for the gamma-distribution-based correction of rate heterogeneity among sites. Two algorithms were used to reconstructMetarhizium...
Di1s.6c7u%ss(i1o/n60) and 1.72% (1/58), respectively; b One sample in the farm was HP-PRRSV and C-PRRSV positive, the positive ratio was 2.04% (1/49); c Two samples in the farm were HP-PRRSV JXA1-R and CP-RPRRRSSVVhpaossiotibves,etshseedpotshiteivpe irgatiiondwuasst3...
According to the classification proposed by [58], all of the studied lakes can be classified as brines due to the predominance of Cl− over SO42− and the ratio of Ca2+ to Na+ (Table 2). Brines are important sources of common salt (NaCl), Ca2+, Mg2+, K+, and other materials,...