4. Freeze in ethanol/dry ice. Store at -70 degrees C. We suggest starting with a molar ratio of 1:1 (insert:vector), with a range of 0.5:1 to 2:1 (insert:vector). The ng quantities should be between 5-10 ng of a 2 kb PCR product in a TOPO cloning reaction.订购...
Too much of the amplification reaction was added to the ligation. The high salt content of PCR reactions can inhibit ligation. Do not use more than 2-3 µl of the PCR reaction in the ligation reaction. Incorrect molar ratio of vector:insert used in...
a1996). The variable FDI is measured as the ratio of FDI inflow to GDP.[translate] ai should be ask myself what i want to do 我应该是问自己什么我想要做[translate] a几年过去了 Several years passed by[translate] aHow were the beaches 怎么是海滩[translate] ...
Photo 4. Vascular thrombosis, infarction, ulceration in colonic mucosa. 图4.血管血栓形成、梗死、结肠粘膜溃疡。 The use of histopathology to verify the typical chronic lesions of the gene-deleted vaccine can be very useful. Pigs that have received those vaccines for ASF will usually have chronic...
The main advantages of using hydrolysis probes are high specificity, a high signal-to-noise ratio, and the ability to perform multiplex reactions. The disadvantages are that the initial cost of the probe may be high, and the assay design may not be trivial. ...
How to use this user manual This user manual is for the following instruments: 24-well PikoReal Real-Time PCR System (Cat. no. TCR0024) and 96-well PikoReal Real-Time PCR System (Cat. no. TCR0096). It aims to give you the information you need for: ● Reviewing safety precautions ●...
a以持续改进寻求企业发展; By improves continually seeks the enterprise to develop;[translate] aThere are many designs of the fuel injection system,which mixes the air and fuel at the correct and most efficient ratio. 有燃料喷射系统的许多设计,混合空气和燃料在正确和多数高效率的比率。[translate] ...
How Does Droplet Digital PCR Work? ddPCR technology uses a combination of microfluidics and proprietary surfactant chemistries to divide PCR samples into water-in-oil droplets (Hindson et al. 2011). The droplets support PCR amplification of the template molecules they contain and use reagents and ...
Too much of the amplification reaction was added to the ligation. The high salt content of PCR reactions can inhibit ligation. Do not use more than 2-3 µl of the PCR reaction in the ligation reaction. Incorrect molar ratio of vector:insert used...
The PCR product generally needs to be diluted since Pfx generates a high concentration of product and using too much insert DNA can hamper the TOPO reaction. A 1:1 molar ratio of vector to insert (or about 2-10ng of insert) is recommended. 1. Use pUC or pUC-based vectors that contain...